We narrowed to 5 results for: shRNA construct

...Production Cloning Cloning your transgene, gRNA, or shRNA of interest into the transfer plasmid can be done...linearized with the restriction enzyme PmeI . This construct is then transformed into AdEasier-1 cells (Addgene...linearized with PacI to create a linear dsDNA construct flanked by ITRs. Cells from the packaging HEK293...expressing E1 are transfected with the linearized construct, and rAdV vectors can be harvested about 7–10 ...purposes. The pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP with the transgene...adding 2.7 kb of packaging space. However, these constructs must be transfected into HER911 E4+ cells for...Ad2 are the most commonly used for rAdV vector construction, due to their high gene transfer efficiency....

...Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs; ~8 kb packaging capacity...Production Cloning Cloning your transgene, gRNA, or shRNA of interest into the transfer plasmid can be done...envelope, and transfer plasmids, and that resulting construct is packaged into a viral particle, it is not possible...

...Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs; 8–10 kb packaging capacity...Production Cloning Cloning your transgene, gRNA, or shRNA of interest into the transfer plasmid can be done...include Cre-lox mice, knockouts using CRISPR or shRNA , and overexpressing of oncogenes and other disease-associated...envelope, and transfer plasmids, and the resulting construct is packaged into a viral particle, it is not possible...

...interested in. Self-replicate — Once you have constructed a plasmid, you can easily make an endless number...frequently accomplished through expression of an shRNA targeting the mRNA of the gene of interest. These...that can drive expression of short RNAs. Mammalian shRNA Tools Genome Engineering Plasmids Used to target...

...deliver genetic cargo into cells. Recombinant AAV constructs are designed by replacing the Rep and Cap regions...Production Cloning Cloning your transgene, gRNA, or shRNA of interest into the transfer plasmid can be done... most commonly used serotype for rAAV vector construction, as it was the first to be well-characterized...host cells. The full-length transgene is later reconstructed within the cell via different mechanisms. One... they form concatemers and splice together, reconstructing the full-length transgene that can then be ...