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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 4 results for: tet off

Showing: 1 - 4 of 4 results

  1. Plan Your Experiment

    Type
    Guide
    ...sites are called off-targets and should be examined during gRNA design. In general, off-target sites are...reagents may be more appropriate. In cases where off-target editing is a major concern, Cas9-gRNA ribonucleoprotein... constitutive (CMV, EF1alpha, CBh) or inducible (Tet-ON); U6 promoter is typically used for gRNA May contain...window of CRISPR component expression may decrease off-target effects Can be used to generate transgenic...components Short window of CRISPR activity may decrease off-target effects Additional Resources: CRISPR protocols...nearby. Select gRNAs based on predicted on-target and off-target activity A PAM sequence is absolutely necessary...using a high-fidelity Cas enzyme. In addition to off-target activity , it is also important to consider...

  2. CRISPR Guide

    Type
    Guide
    ...increase specificity, and decrease off-target effects Sniper-Cas9 - less off-target activity; compatible with...cleaving only one strand of target dsDNA. Off-target effects or off-target activity Cas9 cleavage at undesired...of partial homology throughout the genome, called off-targets, that can impact your experiment. There are...DSB within the target DNA, it’s unlikely that two off-target nicks will be generated close enough to cause... mutating specific amino acid residues to reduce off-target editing. Some mutations disrupt interactions...method, increased fidelity enzymes generate less off-target editing than wild type Cas9. Examples of increased...proofreading and discrimination evoCas9 - decrease off-target effects xCas9 3.7 - mutations in multiple ...

  3. Lentiviral Vector Guide

    Type
    Guide
    ...to the growth medium of the host cells, they kill off any cells that have not incorporated the lentiviral...LEDGF (also known as PSIP1 or p75 ), a lentiviral tethering gene that helps facilitate integration. Despite... of experiments being performed. Your biosafety office can provide more information on your institution's...PMID: 32502836 Johnson, N. M., Alvarado, A. F., Moffatt, T. N., Edavettal, J. M., Swaminathan, T. A., &...30518911 Wells, D. W., Guo, S., Shao, W., Bale, M. J., Coffin, J. M., Hughes, S. H., & Wu, X. (2020). An analytical...

  4. Molecular Biology Reference

    Type
    Guide
    ...reporter gene (for example, luciferase or GFP) that offers a read-out of the activity of the genetic element...high efficiency. Addgene's expanding viral service offers select ready-made AAV and lentiviral particles....England Biolabs F' proA+B+ lacIq ∆(lacZ)M15 zzf::Tn10 (TetR) ∆(ara-leu) 7697 araD139 fhuA ∆lacX74 galK16 galE15.../mL 50 µg/mL Spectinomycin 50 mg/mL 50 µg/mL Tetracycline 10 mg/mL 10 µg/mL *Note: Carbenicillin can be...
Showing: 1 - 4 of 4 results