...maintained and often results in a greater yield of DNA. However, if the plasmid contains a Gateway cassette...plasmids (resulting in a four plasmid system in total). In addition, 3rd generation systems do not use...1 Genome Nuclear Import Is Mediated by a Central DNA Flap. Zennou V, Petit C, Guetard D, Nerhbass U, Montagnier... polypurine tract; recognition site for proviral DNA synthesis. Increases transduction efficiency and ... simple transfections. Is it feasible to express cDNA from a lentiviral transfer plasmids normally used...drive RNA pol III-directed transcription of shRNAs. cDNA expression requires RNA pol II, and thus requires...

...interactions between DNA and protein. Micrococcal nuclease digestion is used to shear the DNA in these assays... uses antibodies to capture protein:DNA complexes. After capture, the DNA is released from the complex...suitable for DNA:histone modifier interactions, which have strong binding between the protein and DNA. Some ...Alternatively, the samples can be normalized to a total protein measurement. Both methods will also serve...light. Shear the samples using sonication to break DNA up into fragments of 300-1000 bps in length. Note...IP section above. Reverse the crosslink, isolate DNA, and perform downstream analysis. Special considerations...gentler but results in non-random fragmentation of the DNA. Controls for capture methods Capture methods should...