1. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

   Type

   Protocol

2. Pouring LB Agar Plates

   Type

   Protocol

3. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

   Type

   Protocol

4. Virus Protocol - Generating Stable Cell Lines

   Type

   Protocol

5. Lentivirus Production

   Type

   Protocol

6. Colony Formation Titering Assay

   Type

   Protocol

7. Fluorescence Titering Assay

   Type

   Protocol

8. General Transfection

   Type

   Protocol

9. AAV Purification by Iodixanol Gradient Ultracentrifugation

   Type

   Protocol

10. AAV Titration by qPCR Using SYBR Green Technology

    Type

    Protocol

11. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type

    Protocol

12. AAV Production in HEK293 Cells

    Type

    Protocol

13. Protocol - How to Perform a Diagnostic Digest

    Type

    Protocol

14. Protocol - How to Run an Agarose Gel

    Type

    Protocol

15. Protocol - Bacterial Transformation

    Type

    Protocol

16. Protocol - How to Create a Bacterial Glycerol Stock

    Type

    Protocol

17. Protocol - How to Ligate Plasmid DNA

    Type

    Protocol

18. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type

    Protocol

19. DNA Quantification

    Type

    Protocol

20. Protocol - How to Inoculate a Bacterial Culture

    Type

    Protocol

21. Protocol - How to Purify DNA from an Agarose Gel

    Type

    Protocol

22. Protocol - How to Streak a Plate

    Type

    Protocol

23. Plasmid Cloning by PCR (with Protocols)

    Type

    Protocol

24. Gibson Assembly Protocol

    Type

    Protocol

25. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

    Type

    Protocol

26. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type

    Protocol

27. Ligation Independent Cloning

    Type

    Protocol

28. What is Polymerase Chain Reaction (PCR)

    Type

    Protocol

29. Protocol - How to Design Primers

    Type

    Protocol

30. Protocol - pLKO.1 – TRC Cloning Vector

    Type

    Protocol