50 results

A wide variety of protocols from Addgene that can be used for basic molecular biology, plasmid cloning, and titering and testing your viral preparations.

Use this protocol to prepare LB agar plates with antibiotic in your lab.

Protocol, tips, and FAQ for how to perform a restriction digest of plasmid DNA

Protocol to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector.

Use this protocol to generate lentivirus

This protocol can be used to titer lentiviral preparations that confer antibiotic resistance.

Protocol that can be used to titer lentiviral preparations carrying a fluorescence marker.

Use this protocol to transfect mammalian cells with your plasmid of interest.

Follow this protocol using Iodixanol gradient ultracentrifugation to get pure adeno-associated virus (AAV) preps for use in your research.

Determine the number of genome-containing particles in an AAV prep using qPCR, SYBR Green Technology, and Addgene’s AAV Titration protocol.

This protocol describes how to generate a monoclonal cell line from a polyclonal pool of stable cells.

Follow this protocol to produce adeno-associated virus (AAV) in HEK293 cells.

Protocol for how to perform a diagnostic digest to verify plasmid size, verify insert orientation, and more.

Standard protocol for performing agarose gel electrophoresis, including tips to improve resolution and separation of bands.

Learn how to transform E. coli with your plasmid of interest.

Protocol for creating a glycerol stock, including additional tips for long-term storage.

Protocol - How to Ligate Plasmid DNA

How to purify plasmid DNA from a bacterial culture, including protocols, tips, and FAQ

Calculate your DNA sample concentration and purity using Addgene’s DNA Quantification Protocol.

Protocol for Inoculating a Bacterial Culture

Protocol for how to purify DNA from an agarose gel, including tips to help improve the resolution and separation of bands.

Learn how to streak bacteria on an LB agar plate to obtain single colonies.

Protocols for plasmid cloning by PCR.

Get started with Gibson Assembly Cloning!

Modify your plasmid MCS or add short sequences using oligo overlap cloning.

Information about plasmid cloning by restriction enzyme digest (subcloning), including design and experimental procedures.

Protocol for Ligation Independent Cloning

Description of Polymerase Chain Reaction with protocol, tips and FAQ

Tips on designing primers for PCR

Addgene's protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting human and mouse genes.

Best practices for performing sequence analysis of a plasmid

Learn how to add antibiotic to a plate after it's already been poured and solidified.

A collection of Addgene's video content, including how-to screen captures, lab procedure protocols, and career advice videos.

Follow this protocol to perform amplification of CRISPR pooled plasmid libraries in Escherichia coli cells.

Follow this protocol to perform digital droplet PCR (ddPCR) for purified AAV titration.

Follow this protocol to perform digital droplet PCR (ddPCR) for lentivirus vector titration.

Follow this protocol to perform RNA extraction without a kit.

In this protocol you will learn the basics of how to weigh reagents.

These guidelines summarize the proper personal protective equipment (PPE) for BSL-1 and BSL-2 labs.

These lab safety guidelines provide steps to ensure you are working in BSL-1 and BSL-2 labs safely.