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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 25 results for: Tat

Showing: 11 - 20 of 25 results

  1. Centrifugation

    Type
    Protocol
    ...that can hold larger containers, but they can also rotate at much higher speeds and are used for more specialized...balanced. Using the centrifuge in an unbalanced state can damage the centrifuge and be dangerous for the...

  2. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...contact with hazardous materials. A sink, eyewash station, safety shower, fire blanket, and extinguisher ... are located before you start. Use the eyewash station if unwanted or biohazardous materials are splashed...

  3. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...that the insert will be added in the correct orientation and prevents the vector from ligating to itself...indicates the various controls: Control Ligase Interpretation Uncut vector - Checks viability of competent...

  4. Pouring LB Agar Plates

    Type
    Protocol
    ...autoclave, you should prepare your plate pouring station: Find an empty section of lab bench with a working...thermometer. Light the flame at the plate pouring station and dilute your antibiotic into your ~60 ℃ molten...

  5. AAV Production in HEK293 Cells

    Type
    Protocol
    ...for 3 h without stirring to allow full precipitation. Precipitation of the viruses can proceed overnight...

  8. Gibson Assembly Protocol

    Type
    Protocol
    ...making a small change in a plasmid (such as point mutations). Generate DNA segments by PCR. Run PCR product...

  9. Colony Formation Titering Assay

    Type
    Protocol
    ...solution through a 0.22 μm filter to remove any precipitates. Stain each well with 1 mL of 0.1% crystal violet...

  10. Pipetting Protocol

    Type
    Protocol
    ...Depending on the pipette you’re using, you will be rotating dials behind the numerical display or the top ...
Showing: 11 - 20 of 25 results