We narrowed to 35 results for: Abo
-
TypeProtocol... working with the volatile reagents in the list above. Procedure Option #1 - Solution D Protocol Before...sure to prepare solution D (see reagent section above for the recipe). If using TRIzol®, jump down to ...
-
Lentivirus ddPCR Titration
TypeProtocol...the titer. Calculations In the experimental setup above, the following virus dilutions were used to transduce...v = Virus volume, mL *In the experimental setup above, the cells per well is 300,000 and the virus volume... -
Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs
TypeProtocol...and bathroom. These items should remain in the laboratory. When traveling between lab spaces, take your... -
Protocol - How to Purify DNA from an Agarose Gel
TypeProtocol...the now gel purified DNA. Find more information about DNA quantification here . Tips and FAQ How do you... -
Plasmid Modification by Annealed Oligo Cloning (with Protocols)
TypeProtocol...water and quantify the concentration (should be about 8ng/μl). Mix the annealed oligos with cut vector... -
Protocol - How to Inoculate a Bacterial Culture
TypeProtocol...suggested amount, instead of the other dry ingredients above. Media without growth (top) and with growth (bottom... -
Molecular Biology Protocol - Restriction Digest of Plasmid DNA
TypeProtocol...more at (Link opens in a new window) NEB's website about star activity . If you are digesting a large number... -
Gibson Assembly Protocol
TypeProtocol... the right). When designing your plasmid, think about what DNA segments you will need to join to create... -
Handling Plasmids from Addgene - Purifying Plasmid DNA
TypeProtocol... supernatant out of the tube if you are worried about losing the pellet. Dry with vacuum or by inverting... -
What is Polymerase Chain Reaction (PCR)
TypeProtocol...manufacturer’s instructions for specific instructions about extension time and temperatures. Initial Denaturation... -
Virus Protocol - Generating Stable Cell Lines
TypeProtocol...cells in the untransduced well (0 µL lentivirus, above) are dying. Perform regular media changes and monitor... -
Plasmid Cloning by PCR (with Protocols)
TypeProtocol...Background In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same ... -
Antibody Validation Using the Indirect ELISA Method
TypeProtocol...point. If your unknown sample’s absorbance falls above the range of the standard curve you will need to... -
AAV ddPCR Titration
TypeProtocol...dilution BSC. Prepare 1X dilution buffer (see recipe above). Save ~25 µL of 1X dilution buffer in a microcentrifuge... -
CRISPR Library Amplification
TypeProtocol...require modifications dictated by the originating laboratory for optimal results. If you obtained the pooled...