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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 30 results for: SET

Showing: 21 - 30 of 30 results

  2. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...AAV-containing Iodixanol solution will flow from the needle set at the 40–60% interface. Make sure that the microcentrifuge...Protocol Video Watch the protocol video below to learn setup and use an iodixanol column gradient for AAV purification...forth a few times to mix the iodixanol that has settled at the bottom of the column. We recommend concentrating...

  3. Western Blot

    Type
    Protocol
    ...follows: Unseal the iBlot 2 PVDF Mini transfer stack. Set the Top Stack to one side and discard the white separator...

  4. Pouring LB Agar Plates

    Type
    Protocol
    ...Place the gel mix in the autoclave and run on a setting that gets the sample to at least 121 ℃ under 20...

  5. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...selection. Use an siRNA selection tool to determine a set of top-scoring targets for your gene. For example...help guide your reaction buffer selection when setting up double digests. Digest pLKO.1 TRC-cloning vector...

  6. CRISPR Library Amplification

    Type
    Protocol
    ...2023 Workflow Timeline Day 1: Transform, recover, set up overnight growth (Estimated time 2-3 hours) Transformation...

  7. General Transfection

    Type
    Protocol
    ... transfected using 1:1, 1:2, 1:3 and 1:6 µg of pRosetta :µg of PEI. The 1:2 and 1:3 ratios provided high...

  8. Lentivirus Production

    Type
    Protocol
    ...were transfected with the GFP-expression plasmid pRosetta using μg total DNA to μg PEI ratios of 1:1, 1:...

  9. Colony Formation Titering Assay

    Type
    Protocol
    ...indicated serial dilutions of the lentiviral vector pRosetta . The following day, cells were treated with puromycin...
Showing: 21 - 30 of 30 results