We narrowed to 30 results for: Vars;

1. Protocol - How to Perform a Diagnostic Digest

   Type

   Protocol

   ...recognize a large range of DNA sequences. Given the variety of these enzymes and the unique sites they recognize...

2. Ligation Independent Cloning

   Type

   Protocol

   ...functions, T4 DNA polymerase can create overhangs of varying length (typically 10-12 bp) based on a specific...

3. Video Library

   Type

   Protocol

   ...Scientific Project Leader Eric Perkins to discuss the various positions he has held in his career, his favorite...

4. Centrifugation

   Type

   Protocol

   ...Depending on the centrifuge their speed ranges can vary, but are typically lower than some other centrifuge...

5. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

   Type

   Protocol

   ...A wide variety of protocols from Addgene that can be used for basic molecular biology, plasmid cloning...

6. Protocol - Bacterial Transformation

   Type

   Protocol

   ... 30-60 secs (45 secs is usually ideal, but this varies depending on the competent cells you are using)...

7. Pipetting Protocol

   Type

   Protocol

   ... stop flowing into the pipette tip. Liquids of various viscosity have different flow rates. The more viscous...

8. Handling Plasmids from Addgene - Purifying Plasmid DNA

   Type

   Protocol

   ...method used, the DNA concentration and purity will vary. For more information on determining DNA concentration...

9. What is Polymerase Chain Reaction (PCR)

   Type

   Protocol

   ...Polymerase (5 units/μL) 36.8 μL Sterile dH 2 O (variable) Note: If you are doing multiple PCRs, save time...

10. CRISPR Library Amplification

    Type

    Protocol

    ...and continue with analysis. “Sufficient” DNA will vary upon intended use. Typically yields range from several...