...tube for 5 min at room temperature on the highest setting. Note: You should see clearly separated layers:...

...AAV-containing Iodixanol solution will flow from the needle set at the 40–60% interface. Make sure that the microcentrifuge...Protocol Video Watch the protocol video below to learn setup and use an iodixanol column gradient for AAV purification...forth a few times to mix the iodixanol that has settled at the bottom of the column. We recommend concentrating...

...follows: Unseal the iBlot 2 PVDF Mini transfer stack. Set the Top Stack to one side and discard the white separator...

...Place the gel mix in the autoclave and run on a setting that gets the sample to at least 121 ℃ under 20...

...selection. Use an siRNA selection tool to determine a set of top-scoring targets for your gene. For example...help guide your reaction buffer selection when setting up double digests. Digest pLKO.1 TRC-cloning vector...

...2023 Workflow Timeline Day 1: Transform, recover, set up overnight growth (Estimated time 2-3 hours) Transformation...

... transfected using 1:1, 1:2, 1:3 and 1:6 µg of pRosetta :µg of PEI. The 1:2 and 1:3 ratios provided high...

...were transfected with the GFP-expression plasmid pRosetta using μg total DNA to μg PEI ratios of 1:1, 1:...

...indicated serial dilutions of the lentiviral vector pRosetta . The following day, cells were treated with puromycin...

...especially in the corner of the well as cells tend to settle there. Once the cells have expanded but before ...