We narrowed to 34 results for: ust

1. Western Blot

   Type

   Protocol

   ...please be aware that the protocol may need to be adjusted to accommodate slight differences between products...determine the optimal dose. Secondary antibodies must match the host species of the primary antibody. ...

2. CRISPR Library Amplification

   Type

   Protocol

   ...significant proportion of the DNA pool. Last Update: August 17, 2023 Workflow Timeline Day 1: Transform, recover...even in the face of a recombined fraction but care must be taken to perform adequate NGS based QC to ensure...

3. Protocol - How to Streak a Plate

   Type

   Protocol

   ... can sterilize it by passing it through a flame, just be sure to allow enough time for the loop to cool...

4. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

   Type

   Protocol

   ...vector. However, the following technique can be used just as easily to add short tags or shRNAs to any vector...

5. Protocol - How to Perform a Diagnostic Digest

   Type

   Protocol

   ...simplest form of diagnostic digest is one in which you just want to verify that the plasmid that you have is...

6. Video Library

   Type

   Protocol

   ...differences in research culture between the US and Australia, and advice for current graduate students considering...

7. Centrifugation

   Type

   Protocol

   ...if it has one) and then close the external lid. Adjust the centrifuge settings according to speed and ...

8. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

   Type

   Protocol

   ...electrophoresis . Tips and FAQ Restriction enzymes MUST be placed in an ice bucket immediately after removal...

9. Colony Formation Titering Assay

   Type

   Protocol

   ...visible in the higher dilutions. Last reviewed on: August 31, 2023...

10. Pipetting Protocol

    Type

    Protocol

    ...view photo of a P1000 pipette shows the volume adjustment ring right below the plunger. This ring changes...

11. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type

    Protocol

    ...and Lamla Thorsten. Human Gene Therapy Methods. August 2015, 26(4): 147-157. doi:10.1089/hgtb.2015.051...

12. Virus Protocol - Generating Stable Cell Lines

    Type

    Protocol

    ...DMEM complete + 10 µg/mL polybrene. Note, this is just a sample of possible dilutions. You may want to ...

13. AAV Titration by qPCR Using SYBR Green Technology

    Type

    Protocol

    ...during initial PCR cycles. This background signal must be removed to accurately determine differences between...

14. Antibody Validation Using the Indirect ELISA Method

    Type

    Protocol

    ...please be aware that the protocol may need to be adjusted to accommodate slight differences between products...