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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 50 results for: CAN

Showing: 41 - 50 of 50 results

  1. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...specifically on selection with carbenicillin; however it can be adapted for any antibiotic through the use of ...liquid is mostly absorbed. The spreading of cells can be done in the same way as the antibiotic, using ...

  2. Ligation Independent Cloning

    Type
    Protocol
    ...polymerase/exonuclease functions, T4 DNA polymerase can create overhangs of varying length (typically 10-...specified distance from their recognition sequence. This can create multiple distinct overhangs with a single ...

  3. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Rev response element (RRE) in the target cells but can be modified for other targets. Primers and a probe...calcium or magnesium, Corning 21-040-CV (cations can affect the attachment of adherent cells) Trypsin ... Prepare 25 ng/µL stocks of each sample. Samples can be used for ddPCR immediately or stored at -20 °C...

  4. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...Deionized water IgG isotype standard 2.5 mg/mL - can use commercial standard or validate an in-house standard...linear trendline for the standards. Google Sheets can calculate this for you as follows: In the Chart Editor...

  5. Western Blot

    Type
    Protocol
    ...proteins. This protocol uses a dry transfer device but can be adapted for wet and semi-dry transfer approaches...rocking platform. Pro-Tip Primary antibody incubation can be reduced to 2 h at RT but may result in more non-specific...

  6. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...Protocols Introduction The AAV Titration protocol can be used to determine the number of genome-containing...usually indicates the presence of primer dimers which can increase background signal and alter the Ct values...

  7. Video Library

    Type
    Protocol
    ...based on size. Following gel electrophoresis, you can cut DNA bands out of the agarose gel and purify the...

  8. Immunocytochemistry

    Type
    Protocol
    ...incubate 30 min at RT in the dark. Pro-Tip The plate can be wrapped in foil to block light. Remove the secondary...

  10. AAV ddPCR Titration

    Type
    Protocol
    ...targeting the ITR elements in the viral vectors but can be modified for other targets. The dilution series...
Showing: 41 - 50 of 50 results