We narrowed to 43 results for: ELL
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TypeProtocol...bacteria, preventing damage to the cell membranes and keeping the cells alive. A glycerol stock of bacteria... and you will not need to obtain more competent cells and retransform. Bacteria on an LB agar plate can...
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Protocol - How to Ligate Plasmid DNA
TypeProtocol...complete plasmid can be transformed into bacterial cells for propagation. The majority of ligation reactions...Interpretation Uncut vector - Checks viability of competent cells and verifies the antibiotic resistance of the plasmid...and vector, and what ratio you want, and it will tell you exactly how much of each to use. Reference Page... -
Using a Light Microscope Protocol
TypeProtocol...like microbes in a drop of pond water or the cells in your cell culture dish. Microscopes come in a huge ...transmission electron microscopes that allow us to see cellular components with science-fiction levels of detail... -
Gibson Assembly Protocol
TypeProtocol...England Biolabs (NEB) (Link opens in a new window) sells DNA Assembly kits and master mixes, including NEBuilder...Assembly (Link opens in a new window) . They also sell ET SSB (Link opens in a new window) . NEB has other... -
Protocol - How to Design Primers
TypeProtocol...another. The size of the primer is very important as well. Short primers are mainly used for amplifying a ... -
Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs
TypeProtocol...clothing from potential spills. Gloves should fit well enough to be snug to your hand, but not so tight... -
Protocol - How to Purify DNA from an Agarose Gel
TypeProtocol... a wider gel comb; or c) loading less DNA in the well. How do you get better separation of bands? If you... -
Protocol - How to Perform Sequence Analysis
TypeProtocol...Sequence Scanner (PC), and Chromas (PC). How can I tell if a peak is mis-called? Open the trace file and... -
Water Bath Protocol
TypeProtocol...restriction digests needed in molecular biology, and thaw cell lines. There are many different types of water baths... -
Protocol - How to Perform a Diagnostic Digest
TypeProtocol...the correct orientation. For detailed protocols as well as tips and tricks see the Restriction Digest and...