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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 45 results for: Ran

Showing: 31 - 40 of 45 results

  1. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ... of Plasmids You may also like... Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture... not need to obtain more competent cells and retransform. Bacteria on an LB agar plate can be stored at...frozen bacteria, preventing damage to the cell membranes and keeping the cells alive. A glycerol stock ...

  2. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ... Inclusion of this information is solely for transparency intended to support reproducibility in science...case, use lower antibody concentrations. The ideal range of the standard curve will vary between targets ...your unknown sample’s absorbance falls above the range of the standard curve you will need to either include...

  3. Kit Free RNA Extraction

    Type
    Protocol
    ...cells. Extract RNA from the homogenized sample(s).Transfer tissue/cell lysate to a 4 mL tube. Add the following...the cold room may damage it. Using a pipettor, transfer the top, aqueous phase to a new RNAse-free tube...

  4. DNA Quantification

    Type
    Protocol
    ...spectrophotometer. A spectrophotometer uses the absorbance/transmission of light through a liquid to determine the concentration...measured under the 260/280 column (A good purity ranges from 1.80-2.00). Repeat for each sample. Notes:...

  6. Using a Light Microscope Protocol

    Type
    Protocol
    ...your cell culture dish. Microscopes come in a huge range of shapes and sizes - from phone-sized, foldable...powerful (but are cheap and accessible) to massive transmission electron microscopes that allow us to see cellular...

  7. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...the solution has a tendancy to boil over. Placing saran wrap over the top of the flask can help with this...are usually referred to as ‘bands’ due to their appearance on the gel. Pro-Tip If you will be purifying ...

  8. Pouring LB Agar Plates

    Type
    Protocol
    ...x (0.220 L) = 8.14 g pre-mixed LB-agar powder. Transfer the LB-agar powder you’ve measured out into an...molten agar from boiling over in the autoclave. Transfer the sterile water (in our case 220 mL) to the ...

  9. Protocol - How to Streak a Plate

    Type
    Protocol
    ...also like... Making LB Agar Plates Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture...
Showing: 31 - 40 of 45 results