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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 47 results for: TOR

Showing: 31 - 40 of 47 results

  1. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ... mM EDTA Store Solution I at 4°C Solution II - Denaturing Solution 0.2 N NaOH 1.0% SDS Store Solution ...acetate 23 mL glacial acetic acid 57 mL of dH 2 O Store Solution III at 4°C Grow 2 mL overnight cultures... room temperature before quantifying and using. Store DNA at 4°C. Tips and FAQ Plasmid purification kits...

  2. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...sites. Often, the size of the plasmid insert and vector backbone are known and thus this technique can ...Restriction Digest If you clone an insert into a vector by a single restriction enzyme, you will need to...which orientation the insert is ligated into the vector backbone and therefore the only thing left to do...

  4. Immunocytochemistry

    Type
    Protocol
    ...water Microscope slide Anti-fade mounting medium Laboratory wipes 15 mL conical tubes 50 mL conical tubes...paraformaldehyde and follow your institution's laboratory safety guidelines for disposing of waste in the...remove the coverslip. Blot the coverslip with a laboratory wipe to remove excess liquid. Add 1 drop of anti-fade...

  5. Using a Light Microscope Protocol

    Type
    Protocol
    ...research and are found in many different types of laboratories. These tools allow you to observe specimens ... in the future. Conclusion Like any important laboratory instrument, you should be sure to take care of... up your work area and microscope after use and store the microscope appropriately....

  6. Pouring LB Agar Plates

    Type
    Protocol
    ...your plates are ready for use or storage at 4 ℃. You should not store your plates for longer than 1 month...material to reduce condensation. The plates are then stored at 4 ℃ until use. Once your plates have solidified...

  7. Protocol - How to Streak a Plate

    Type
    Protocol
    ...loop Bunsen burner (or other small flame source) Incubator Marker Reagents LB agar plate (with appropriate...resistance and your initials. Labeling within a laboratory setting is important for organization, and it...

  8. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...spreading of antibiotic makes it easy for an investigator to conveniently plate and select transformed...spreading Bunsen burner (or other small flame source) Incubator Reagents 6 cm diameter LB/agar plates without ...

  9. Water Bath Protocol

    Type
    Protocol
    ... lab! Introduction A water bath is a piece of laboratory equipment that helps bring your materials to .... Water baths can also be placed in walk-in refrigerators to achieve a temperature between 4°C and room...

  10. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...a blank chart to your spreadsheet. In the Chart Editor , select the Chart Type drop down menu and select...calculate this for you as follows: In the Chart Editor , select the Customize tab. Select the Series drop...
Showing: 31 - 40 of 47 results