Coomassie Purity Stain of Recombinant Antibodies
Type
Protocol
... band. Use the line tool to connect the bottom of each peak. Select the wand tool. Fill in each peak by...known concentration. After staining with Coomassie blue, protein band intensities are measured using ImageJ...lane of the gel as follows: Figure 2 Using the box tool, draw a box around the entire first gel lane (as...ready-to-use bio-safe Coomassie G-250 stain (Addgene uses SimplyBlue SafeStain) and ImageJ software. The sample...ImageJ software and a standard curve is generated. The ratio of the antibody protein content to the total...reagents, and methods that we use in our lab at Addgene. Equipment and reagents from other vendors should...accommodate slight differences between products. Addgene does not endorse or recommend specific products...