We narrowed to 2 results for: competent cells/1000

1. Protocol - Bacterial Transformation

   Type

   Protocol

   ...commonly referred to as 'competent cells.' Many companies sell competent cells, which come frozen and are...instructions that came with your competent cells. Pro-Tips Commercial competent cells range significantly in their... or SOC media Competent cells DNA you'd like to transform Procedure Take competent cells out of -80°C ...to use electro-competent cells. Instead of relying on the heat-shock to cause the cells to take up the...DNA, especially when using highly competent cells. If you used 100-1000 ng of total DNA in a ligation you... save money, many labs also make their own competent cells. This is a relatively simple procedure and ... (usually 10 pg - 100 ng) into 20-50 μL of competent cells in a microcentrifuge or falcon tube. GENTLY...

2. CRISPR Library Amplification

   Type

   Protocol

   ...obtained. Ensure that electrocompetent cells are being used. Chemically competent cells will not provide adequate...Alternatives include Stbl4 cells or other ultra-high efficiency electrocompetent cells that are suitable for...limited. Day 2: Harvest cells and purify DNA (Estimated time 3-4 hours) Cells should be harvested first...HiSpeed Max, Catalog #12663) Tips (1000 µL, 200 µL, 10 µL) Bacti Cell Spreaders (VWR, 60828-680) 5 mL and...Maxipreps rely on incremental, ordered cell lysis. E. coli cells are subject to lysis by freeze-thaw if...MicroPulser TM , Bio-Rad 1652100) Reagents 200 µL electrocompetent cells (Default: 4 tubes of Endura Duos, Lucigen... or recombination-prone DNA. The use of electrocompetent cells is essential to ensure high efficiency ...