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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3 results for: crispr c plasmids

Showing: 1 - 3 of 3 results

  1. CRISPR Library Amplification

    Type
    Protocol
    ...amplification of CRISPR pooled plasmid libraries in Escherichia coli cells....starting point for CRISPR libraries. This protocol allows the amplification of a pooled-plasmid library in Escherichia...differences, and plasmid recombination can all impact the representation of individual plasmids in the pooled... ). Lentiviral plasmids can recombine between their LTRs resulting in a smaller plasmid containing only...media at 37 °C (for at least 15 minutes). Prewarm 3X LB Agar + Antibiotic plates at 37 °C. Prewarm 8X ... Protocols CRISPR Library Amplification...Amplification CRISPR Library Amplification You may also like... Pooled libraries Guide to Using Pooled Libraries...

  2. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...Addgene plasmid #52962 ) and is described in Improved vectors and genome-wide libraries for CRISPR screening...heat-inactivated FBS and 5mL of 100X glutaGRO. Store at 4 °C. Pro-Tip Different brands and FBS lots can promote...it can be inactivated in the lab by heating to 56 °C for 30 min. Considerations Before You Start The health...dose of antibiotic, which may not be stable at 37 °C. To achieve a stable cell pool, the antibiotic selection...well plate and left undisturbed for 13 days. (a, b, c) Colonies formed by expansion of single cells for ...

  3. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...Addgene plasmid #52962 ) and is described in Improved vectors and genome-wide libraries for CRISPR screening...heat-inactivated FBS and 5 mL of 100X glutaGRO. Store at 4 °C. Polyclonal stable cell pool: see our protocol for...
Showing: 1 - 3 of 3 results