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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 6 results for: ena/1000

Showing: 1 - 6 of 6 results

  1. AAV Production in HEK293 Cells

    Type
    Protocol
    ...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette Hemocytometer or cell ...system, Nalgene, 565-0010 (or for larger volumes 1000 mL 0.45 µm Rapid-Flow PES Filtration Unit, Nalgene...solution through a 0.22 μm membrane. Aliquot 500–1000 μL into sterile tubes. Store the tubes at -80 °C... deionized water and adjust to a final volume of 1000 mL. Stir at RT until fully dissolved. Pro-Tip This...cells. Pass the cells twice a week during the maintenance phase and do not allow cells to reach 100% confluence...

  2. AAV ddPCR Titration

    Type
    Protocol
    ...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette 1–10 µL multichannel pipette...calculate the titer for each sample: $$T = {R*C*D*1000\over V}$$ Where: T = Titer, GC/mL R = Reaction volume...min) Ramp Rate (°C/sec) # Cycles Denaturation 95 10 2 1 Denaturation 95 0.5 2 50 Annealing/Extension 60...

  3. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette Pipette controller Microcentrifuge...Pipette tips, 200 µL, VWR 76322-150 Pipette tips, 1000 µL, VWR 76322-154 Pipettes, 5 mL, VWR 89130-896 ...and ImageJ software. The sample is separated by denaturing polyacrylamide gel electrophoresis alongside ...

  4. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator...generating cell lines using lentiviral vectors enables long-term protein expression studies. Moreover,...resistance (e.g., puromycin, blasticidin), which enables selection of a stable cell culture after transduction...Performing antibiotic selection on transduced cells enables elimination of untransduced cells, resulting in...

  5. Pipetting Protocol

    Type
    Protocol
    ...middle is hundreds, and the third is tens. Therefore, 1000 µL would read as 100 from top to bottom (as shown...molecular biology. You’ll use a pipette in many scenarios to accurately dispense small amounts of liquid...Anatomy of a Pipette This front view photo of a P1000 pipette shows the plunger at the top, which is used...tip off of the pipette. This side view photo of a P1000 pipette shows the volume adjustment ring right below...come in standard sizes: P2, P10, P20, P200, and P1000. The table shows the pipette with the appropriate...This image shows the P2, P10, P20, P100, P200, and P1000 as shown from left to right. Pipette Dispense Volume... 2 to 20 µL P100 10 to 100 µL P200 20 to 200 µL P1000 100 to 1,000 µL Each pipette should be used with...

  6. Lentivirus ddPCR Titration

    Type
    Protocol
    ...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette 2–50 µL multichannel pipette...°C/sec) # Cycles Enzyme Activation 95 10 2 1 Denaturation 94 0.5 2 40 Annealing/Extension 60 1 2 40 Enzyme...
Showing: 1 - 6 of 6 results