We narrowed to 5 results for: nms

...0.5 g BSA and 150 µL Triton X-100 in 50 mL PBS. 300 nM DAPI working solution: Prepare a 300 µM DAPI stock... to 100 µL PBS. Protect from light. Prepare a 300 nM DAPI working solution by diluting 5 µL of the 300...(Optional) Counterstain nuclei with 500 µL of 300 nM DAPI working solution for 10 min at RT in the dark...

...) 1 µL 9 µL 900 nM, 250 nM 20X RPP30 primers/probe (HEX/VIC) 1 µL 9 µL 900 nM, 250 nM Nuclease-free water...

... (FAM) 0.5 µL 4.5 µL 250 nM Forward ITR Primer (10 µM) 1.8 µL 16.2 µL 900 nM Reverse ITR Primer (10 µM...µM) 1.8 µL 16.2 µL 900 nM Nuclease-free water 5.9 µL 71.55 µL Total Volume 20 µL Vortex the master mix...

...reaction. Measure the absorbance of each well at 450 nm on the spectrophotometer. Use the spectrophotometer...stopped after 15 minutes. Absorbance was read at 450 nm and plotted against the micrograms of antigen loaded...

...30 min at 37 °C . Determine the absorbance at 590 nm . Calculate the average absorbance of the duplicate...