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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 80 results for: nms

Showing: 1 - 20 of 80 results

  1. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Photoswitchable Timers Blue/UV Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids...Expression Jump to Top Cyan Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids...Expression Jump to Top Green Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids...Expression Jump to Top Yellow Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids...Expression Jump to Top Orange Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids...Expression Jump to Top Red Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids...Expression Jump to Top Far-Red Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids...

  2. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ... of light at three key wavelengths: 230 nm, 260 nm, and 280 nm. Nucleic acids exhibit a characteristic...absorption occurs below 230 nm, with a distinct secondary peak around 280 nm thanks to aromatic amino acids...your sample's absorption spectra from 260 nm towards 270 nm. If your purification protocol uses phenol...absorption peak occurs near 260 nm as expected — if the peak is closer to 270 nm, you may have phenol in your...range of wavelengths with maximal absorption at 260 nm (Figure 1A). Thanks to the Beer–Lambert law, the ...concentration based on the sample’s absorption at 260 nm (A260). Figure 1: Characteristic absorption.... These other molecules can alter the overall 260 nm reading, distorting the final concentration calculation...

  3. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    ... mTFP1 with a 445/30 nm excitation filter, a 470 nm beamsplitter, and a 495/30 nm emission filter. This...Its excitation maxima is at 506 nm and its emission maxima is at 517 nm. Because mNeonGreen wavelengths...excitation and emission maxima were red-shifted (462 nm and 492 nm, respectively), they gave this a teal classification...emission peaks (max excitation at 493 nm and max emission at 509 nm), making it useful in multicolor labeling...(excitation maximum 399 nm) because it does not significantly excite at 400 nm while EGFP does. Find mWasabi...proteins that were photostable and had a high 480/530 nm emission ratio. They arrived at a protein which had...

  5. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...wavelengths below 380 nm lie within the UV spectrum, while wavelengths over 700 nm make up the infrared...Ultraviolet (355 nm)   BD Horizon™ BUV737 BD Horizon™ BUV395   Violet (405 nm) BD Horizon™...Yellow/Green (561 nm) PEBD Horizon™ PE-CF594PE-Cy5PE-Cy7       Red (640 nm)   APCAlexa ...local maximum at just under 500 nm and a global maximum at around 560 nm, leading to different levels of...excites at 640 nm (Table 1), while comparing the two fluorophore's excitation spectra around 640 nm (Figure ...maximum lies around 650 nm, APC-Cy7's global excitation maximum is about 750 nm. If we were to use a laser...absorption at 650 nm, APC-Cy7's optimal excitation happens at higher wavelengths of about 750 nm. Source: https...

  6. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...separated by 50-60 nm. For example, CFP (ex 430 nm / em 474 nm) and YFP (ex 514 nm / em 527 nm) can be imaged...imaged together but CFP and GFP (ex 488 nm / em 507 nm) show some crosstalk between the two fluorescent proteins... combination on a confocal (405 / 488 / 561 / 640 nm). In our hands the best fluorescent proteins for ...

  7. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ... exciting GFP-labeled plant cells at 405 nm instead of 488 nm, Sattarzadeh et al. noticed that GFP had...green state after excitation at 488 nm, photoconverted with 405 nm light, and then captured in the red...red state after excitation at 561 nm. This imaging process worked well with the common GFP variants EGFP...no red color was observed after excitation at 488 nm, indicating that spontaneous conversion did not occur...with other FPs since its emission wavelength (612 nm) is distinct from other commonly used FPs, and GFP...

  8. Bacterial Expression Systems

    Type
    Collection
    ...Red light (660 nm) Escherichia coli Heikki Takala 31395 pJT118 PcpcG2 Green Light (532 nm) Escherichia ...Dominic Esposito 43796 pDawn FixK2 Blue light (470 nm) Escherichia coli Andreas Moeglich 68940 pRMC2 Pxyl...Cheryl Arrowsmith 43795 pDusk pR_FixK2 Blue light (470 nm) Escherichia coli Andreas Moeglich 26092 pET15-MHL...Nandicoori 188974 pREDusk-AmpR-MCS FixK2 Red light (660 nm) Escherichia coli Heikki Takala 47641 pCS-PluxIlux...

  9. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ... - 467 nm Cyan 474 - 492 nm Green 499 - 519 nm Yellow 524 - 538 nm Orange 559 - 572 nm Red 574... 574 - 610 nm Far-Red 625 - 659 nm Infra-Red ≥ 670 nm In addition to emission wavelength range,...

  10. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ...650 nanometers (nm), and the absorbance of water, which absorbs wavelengths above 900 nm or so. In other...other words, wavelengths between 650 nm and 900 nm will pass through animal and human tissues largely unimpeded...fluorescent proteins that operate within the 750 to 800 nm range, and his team is also looking in the direction...

  11. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ..., Keima emits at 620 nm. The ratio of signal strength excited at 586 nm to 440 nm serves as a measure ...dimeric Keima is excited at 440 nm and in acidic environments at 586 nm. Regardless of excitation wavelength...

  12. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...green fluorescence when exposed to 500 nm light.  Under 400 nm cyan light, Dronpa dimers dissociate, Dronpa...2 minute pulse of 500 nm light (turn on psRaf1) followed by 3 seconds of 400 nm light (turn off psRaf1...photoswitchable MEK1 is called psMEK1. When exposed to 500 nm violet light, the Dronpa dimers interact and lock...incubated with an inhibitor of choice, exposed to 500 nm cyan light to activate psMEK1, and the localization...

  13. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    ... absorb maximally at 260 nm. Proteins on the other hand absorb best at 280 nm and organic compounds and...and chaotropic salts maximally absorb at 230 nm. The A260/A280 ratio is used as an indicator of DNA purity...forms a blue complex that can be quantified at 595 nm. This method is time consuming and has low sensitivity...

  16. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...absorbance maximum around 488 nm and it emits light that peaks at 508 nm. The protonated state, however...resembles a cylinder with a length of 4.2 nm and a diameter of about 2.4 nm (Hink et al., 2000). The complete ...however, does not absorb light at 488 nm. The ratio between the two states is reflected by the pKa, which ...

  19. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ... proteins in whole animals, light at or above 600 nm wavelengths should be used. Researchers often use...emit at near optimal wavelengths (Fluc emits at 610 nm) and do not require external light sources. Unfortunately...whole-animal imaging, however NLuc’s emission peak is at 460 nm thus making it non-optimal for in vivo imaging. The...light at a tissue-penetrating wavelength above 600 nm. LumiFluors Schaub, F.X., et al. (3) designed two...being the brightest since it had an emission near 600 nm – a clear demonstration of why a BRET reporter can...

  20. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...wtGFP’s excitation spectrum- a major 397 nm peak and minor 475 nm peak, respectively. When wtGFP is illuminated...illuminated with UV-violet light (~400 nm), the chromophore photoconverts predominantly to the anionic form...in Fluorescence) Brightness Excitation/ Emission (nm) PA-GFP Monomer UV-Violet Dark/ Green ~200 Medium...in Fluorescence) Brightness Excitation/ Emission (nm) Dronpa Monomer UV-Violet Dark/Green N/A (high...
Showing: 1 - 20 of 80 results