Adenoviral Plasmids
Adenoviral vectors are widely used for gene delivery due to their high packaging capacity, high efficiency in driving strong transient gene expression in both dividing and non-dividing cells, and relative ease of large-scale production.
In order to produce recombinant adenoviral vectors, researchers generally need to use two plasmids:
- A shuttle plasmid (or transfer plasmid) containing the transgene of interest, the ITRs, and homology regions for recombination with the adenoviral plasmid.
- An adenoviral plasmid containing the rest of the adenoviral genome necessary for viral production and homology regions for recombination with the shuttle plasmid.
This collection provides a curated selection of popular plasmids that can be used for adenoviral vector production. Read our adenoviral vector guide for more information about adenoviral components, generations, viral production, and viral safety.
Shuttle Plasmids
Browse commonly used shuttle plasmids for adenoviral vector production. Want a plasmid with your gene of interest? Search our database for shuttle plasmids with inserts.
| ID | Plasmid | Description | PI | |
|---|---|---|---|---|
| 16402 | pShuttle | For production of viruses containing transgene under a chosen promoter | Vogelstein | |
| 16403 | pShuttle-CMV | For production of viruses containing transgene under CMV promoter | Vogelstein | |
| 16404 | pAdTrack | For production of GFP-trackable viruses containing transgene under a chosen promoter | Vogelstein | |
| 16405 | pAdTrack-CMV | For production of GFP-trackable viruses containing transgene under CMV promoter | Vogelstein | |
| 50957 | RedTrackCMV | For production of mRFP-trackable viruses containing transgene under CMV | Bamburg | |
| 16407 | pAdEasy 2-GFP beta-gal | Test plasmid that contains β-gal and GFP and a ∼10 Kb genomic DNA stuffer | Vogelstein | |
| 18703 | pEZYshuttle | Modified pShuttle-CMV (Addgene #16403) plasmid with Gateway cassette | Zhang | |
| 50958 | ShuttleNSE | For production of viruses containing transgene under Neuron-specific enolase (NSE) promoter | Bamburg | |
| 62621 | pShuttle-CMV-F2A-T2A-Venus | For production of viruses with multicistronic expression and a Venus tag | Wang | |
| 50959 | ShuttleMCP | For production of viruses containing transgene under mouse cofilin promoter (MCP) | Bamburg | |
| 62622 | pShuttle-CMV-F2A-T2A | For production of viruses with multicistronic expression | Wang |
Adenoviral Strains
Browse bacterial strains for adenoviral vector production.
| ID | Strain | Description | PI | |
|---|---|---|---|---|
| 16398 | BJ5183 | E. coli strain for recombination between shuttle plasmid and adenoviral plasmid | Vogelstein | |
| 16399 | AdEasier-1 | BJ5183 derivative that contains the pAdEasy-1 (Addgene #16400) plasmid | Vogelstein |
Other Resources
Resources on addgene.org
Protocols
Content last reviewed: 12 November 2025
Do you have suggestions for other plasmids that should be added to this list?
Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource!