Open Source Wnt Project
The Open Source Wnt Project was initiated to create a collection of standardized Wnt plasmids that are easily accessible to the research community. The project and plasmids were created by Dr. Marian Waterman's group at UC Irvine and Dr. David Virshup's group at Duke Singapore. These plasmids have been described in:
A uniform human Wnt expression library reveals a shared secretory pathway and unique signaling activities. Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, Waterman ML. Differentiation. 2012 Sep;84(2):203-13. PubMed 22784633.
In addition to this original set of 76 clones, Dr. Bryan MacDonald and Dr. Xi He have contributed a modified version of each tagged Wnt that enables epitope tagging without loss of Wnt signaling activity.
The kit is $375, plus shipping and handling. This kit consists of 95 plasmids, and will be shipped as bacterial glycerol stocks, 96-well format, on dry ice. Samples should be frozen at -80 degrees Celsius immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs.
The Wnt plasmids fill 95 wells on a single 96-well plate.
Note that Addgene uses UPS for shipments and can ship dry ice to most countries, excluding China. Scientists from China interested in obtaining this kit should contact firstname.lastname@example.org.