Addgene - scramble shRNA Plasmid Data

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pLKO.1 - TRC cloning vector

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Plasmid 1864: scramble shRNA

Gene/insert name:		scramble
Insert size (bp):		60
Vector backbone:		pLKO.1 (Search Vector Database)
Backbone manufacturer:		Stewart SA, RNA 2003 Apr; 9(4):493-501.
Type of vector:		Lentiviral,RNAi
Backbone size (bp):		7032
Cloning site 5':		AgeI
Site destroyed during cloning:		Yes
Cloning site 3':		EcoRI
Site destroyed during cloning:		No
5' Sequencing primer:		LKO.1 5' (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		Don't Know
Grow in standard E. coli @ 37C:		No
Please specify bacterial strain for growth and growth condition:		XL10-Gold Ultracompetent Cells from Stratagene. 37oC.
Selectable markers:		Puromycin
Sequence:		View sequence
Principal Investigator:		David M. Sabatini
Additional Documents:		Addgene's pLKO.1 protocol (HTML)
Terms and Licenses:		MTA

Comments: shRNA for use as a negative control. Sequence of hairpin is: CCTAA GGTTA AGTCG CCCTC GCTCT AGCGA GGGCG ACTTA ACCTT AGG. For packaging, please use pCMV-dR8.2 dvpr (Addgene plasmid #8455) and pCMV-VSVG (Addgene plasmid #8454).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Sarbassov DD et al. (Science 2005 Feb 18;307(5712):1098-101. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 1864" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.