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viral-icon-01.png Viral Service: Viviana Gradinaru PHP AAV Serotypes


As part of our Viral Service, Addgene is producing ready-to-use viral preparations in the PHP.eB, PHP.S, and PHP.V1 serotypes from the Viviana Gradinaru laboratory and the Caltech CLOVER Center. As part of our standard viral production, these viral vectors undergo quality control, including AAV titering by SYBR green qPCR, in vitro and (when possible) in vivo testing, and full sequencing of the final viral vector preparation.

PHP.eB

These viral vector preparations were produced with the pUCmini-iCAP-PHP.eB plasmid (Addgene #103005).

The PHP.eB serotype exhibits efficient transduction of the central nervous system via systemic delivery in adult animals. In vivo studies showed that, compared to PHP.B and AAV9, intravenous injection of PHP.eB AAV led to an increase in both the number of transduced cells and the expression level per cell. In vivo, PHP.eB transduced the majority of neurons in the cortex and striatum, and over 75% of cerebellar Purkinje cells.

Note on PHP.eB tropism and specific mouse lines: Enhanced CNS tropism exhibited by the PHP.eB serotype has been reported to occur through the cellular receptor LY6A (DOI 538421). For information on whether the PHP.eB serotype will work in your mouse line, see Supplementary Table 3.

Browse Available PHP.eB AAV

ID Name Promoter Description Category PI
Controls
28306 pAAV-FLEX-tdTomato CAG tdTomato, Cre-dependent Control Boyden
37825 pAAV-CAG-GFP CAG GFP Control Boyden
59462 pAAV-CAG-tdTomato CAG tdTomato Control Boyden
104061 CAG-NLS-GFP CAG NLS-GFP Control Gradinaru
Chemogenetics
44361 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Syn1 Activator, Cre-dependent DREADD Roth
44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Syn1 Inhibitor, Cre-dependent DREADD Roth
Biosensors
104491 pGP-AAV-syn-FLEX-jGCaMP7s-WPRE Syn Calcium sensor, Cre-dependent GCaMP Kim
104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE Syn Calcium sensor, Cre-dependent GCaMP Kim
Optogenetics
26973 pAAV-hSyn-hChR2(H134R)-EYFP Syn Activator ChR2 Deisseroth
20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA EF1a Activator, Cre-dependent ChR2 Deisseroth
Recombinases
105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn Cre-EGFP expression Cre Wilson

PHP.S

These viral vector preparations were produced with the pUCmini-iCAP-PHP.S plasmid (Addgene #103006).

The PHP.S serotype exhibits efficient transduction of the peripheral nervous system via systemic delivery in adult animals. In vivo studies showed that, relative to parent capsid AAV9, intravenous injection of PHP.S AAV led to an increase in both the number of transduced cells and the expression level per cell. PHP.S AAV was also shown to transduce neurons in the enteric nervous system and in other peripheral ganglia, such as the cardiac ganglia. When used with Cre transgenic lines or cell-type-specific promoters, this serotype could enable targeted transduction of subsets of cells within these regions.

Browse Available PHP.S AAV

ID Name Promoter Description Category PI
28306 pAAV-FLEX-tdTomato CAG tdTomato, Cre-dependent Control Boyden
59462 pAAV-CAG-tdTomato CAG tdTomato Control Boyden

PHP.V1

These viral vector preparations were produced with the pUCmini-iCAP-PHP.V1 plasmid (Addgene #127847).

The PHP.V1 serotype exhibits efficient transduction of vesicular brain cells via systemic delivery in adult animals. In vivo studies showed that, compared to PHP.eB and AAV9, intravenous injection of PHP.V1 AAV led to a >40% increase in transduction of cortical brain vasculature. PHP.V1 AAV was also shown to transduce S100+ astrocytes, but is less efficient in astrocyte transduction than PHP.eB when used with an astrocyte specific promoter.

Browse Available PHP.V1 AAV

ID Name Promoter Description Category PI
104052 pAAV-CAG-DIO-EYFP CAG EYFP, Cre-dependent Control Gradinaru

Citation Information

When using the PHP serotypes in future publications, please acknowledge Viviana Gradinaru and cite Chan et al., Nat Neurosci, 20(8):1172-1179. Pubmed.

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Please let us know what you would like to see available in viral format. Please note this does not guarantee viral service, but lets us know what viruses would be helpful to the scientific community.

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