pAF256
(Plasmid #105496)

• Purpose: pRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and LgBiT
• Depositing Lab: Wiep Klaas Smits
• Publication: Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(18)31160-4. doi: 10.1016/j.jmb.2019.01.001.

Backbone

• Vector backbone: pRPF185
• Backbone manufacturer: R.P. Fagan, University of Sheffield
• Total vector size (bp): 8076
• Vector type: ATc-dependent expression in C. difficile

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: HupA-SmBiT/LgBiT
• Species: C. difficile / synthetic
• Insert Size (bp): 900
• Promoter: Ptet

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CTGGACTTCATGAAAAACTAAAAAAAATATTG
• 3′ sequencing primer: CACCGACGAGCAAGGCAAGACCG

Resource Information

• Supplemental Documents:
  • Addgene-pAF256.gb
  • Geneious file of plasmid pAF256
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/09/27/426809 for bioRxiv preprint.