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pAAV-CBh-mKate2-IRES-MCS (C-gap-WT)
(Plasmid #105922)


Item Catalog # Description Quantity Price (USD)
Plasmid 105922 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

* Login to view industry pricing.


  • Vector backbone
  • Modifications to backbone
    11bp deletion (aaagcccgggc) in left ITR removes 1 SmaI site
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number

Gene/Insert 1

  • Gene/Insert name
  • Mutation
    codon optimized
  • Promoter CBh

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pCBhProF2 (5-agggtttaagggatggttgg)
  • 3′ sequencing primer pCDH-rev (5-GCATTCCTTTGGCGAGAG)
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    IRES-Multiple cloning site
  • Alt name
    MCS is FseI-PacI-SpeI-BamHI-XhoI-StuI-MluI-NheI-BmtI-AsiSI-AscI
  • Promoter same CBh promoter as mKate2

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site N/A (unknown if destroyed)
  • 3′ cloning site N/A (unknown if destroyed)
  • 5′ sequencing primer IRES-F (5-TGGCTCTCCTCAAGCGTATT)
  • 3′ sequencing primer WPRE-R (5-CATAGCGTAAAAGGAGCAACA)
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Entire transgene cassette can be removed with NotI/XbaI digest; CBh promoter can be removed by XbaI/AgeI digest; mKate2 can be removed with SaI/EcoRI digest; IRES can be removed by EcoRI/FseI digest

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CBh-mKate2-IRES-MCS (C-gap-WT) was a gift from Marcella Patrick (Addgene plasmid # 105922 ; ; RRID:Addgene_105922)