pCAGGS-mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2
(Plasmid
#128167)
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PurposeMonomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, GSAx9 linker)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 128167 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAGGS
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Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2
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SpeciesSynthetic
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bypCAGGS is from BCCM/LMBP. mTagBFP2 is from Michael Davidson. sfGFP is from Sandia Biotech. iCre is from Rolf Sprengel. ERT2 is from Pierre Chambon.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Linker between sfGFP and iCre-ERT2 is a clean GSAx9 sequence, similar in length to the mutated P2A sequence in the plasmid pCAG-sfGFP-iCre-ERT2 (#125749).
The sfGFP fluorescence from the sfGFP-iCre-ERT2 is dim, as described in Nam and Capecchi, 2020 (https://doi.org/10.1186/s13064-020-00139-5) and a Figshare page (https://doi.org/10.6084/m9.figshare.13218437.v35) In short, it is not visible to the eyes, but can be detected with flow cytometry.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAGGS-mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2 was a gift from Mario Capecchi (Addgene plasmid # 128167 ; http://n2t.net/addgene:128167 ; RRID:Addgene_128167) -
For your References section:
Lrig1 expression prospectively identifies stem cells in the ventricular-subventricular zone that are neurogenic throughout adult life. Nam HS, Capecchi MR. Neural Dev. 2020 Mar 17;15(1):3. doi: 10.1186/s13064-020-00139-5. 10.1186/s13064-020-00139-5 PubMed 32183906