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pPB-C6F/TdTomato
(Plasmid #140826)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 140826 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTRE-Tight-BI
  • Backbone manufacturer
    Clontech
  • Total vector size (bp) 16425
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    OCT4
  • Alt name
    POU5F1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1080
  • GenBank ID
    NM_002701.6
  • Entrez Gene
    POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)

Gene/Insert 2

  • Gene/Insert name
    SOX2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    954
  • GenBank ID
    NM_003106.4
  • Entrez Gene
    SOX2 (a.k.a. ANOP3, MCOPS3)

Gene/Insert 3

  • Gene/Insert name
    KLF4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1410
  • GenBank ID
    NM_004235.6
  • Entrez Gene
    KLF4 (a.k.a. EZF, GKLF)

Gene/Insert 4

  • Gene/Insert name
    C-MYC
  • Alt name
    MYC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1320
  • GenBank ID
    NM_002467.6
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)

Gene/Insert 5

  • Gene/Insert name
    KLF2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1065
  • Mutation
    L104P- please see depositor comments
  • GenBank ID
    NM_016270.4
  • Entrez Gene
    KLF2 (a.k.a. LKLF)

Gene/Insert 6

  • Gene/Insert name
    NANOG
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    918
  • Entrez Gene
    NANOG

Gene/Insert 7

  • Gene/Insert name
    tdTomato
  • Insert Size (bp)
    1431

Resource Information

Depositor Comments

Depositor confirms the L104P mutation in KLF2 does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB-C6F/TdTomato was a gift from Hideyuki Okano (Addgene plasmid # 140826 ; http://n2t.net/addgene:140826 ; RRID:Addgene_140826)
  • For your References section:

    Naive-like ESRRB(+) iPSCs with the Capacity for Rapid Neural Differentiation. Kisa F, Shiozawa S, Oda K, Yoshimatsu S, Nakamura M, Koya I, Kawai K, Suzuki S, Okano H. Stem Cell Reports. 2017 Dec 12;9(6):1825-1838. doi: 10.1016/j.stemcr.2017.10.008. Epub 2017 Nov 9. 10.1016/j.stemcr.2017.10.008 PubMed 29129686