pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro Citations (26)
Originally described in: A SARS-CoV-2 protein interaction map reveals targets for drug repurposing.Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J, Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, Shoichet BK, Krogan NJ Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. Epub 2020 Apr 30. PubMed Journal
Articles Citing pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro
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| A Saliva-Based RNA Extraction-Free Workflow Integrated With Cas13a for SARS-CoV-2 Detection. Azmi I, Faizan MI, Kumar R, Raj Yadav S, Chaudhary N, Kumar Singh D, Butola R, Ganotra A, Datt Joshi G, Deep Jhingan G, Iqbal J, Joshi MC, Ahmad T. Front Cell Infect Microbiol. 2021 Mar 16;11:632646. doi: 10.3389/fcimb.2021.632646. eCollection 2021. PubMed |
| Recurrent emergence of SARS-CoV-2 spike deletion H69/V70 and its role in the Alpha variant B.1.1.7. Meng B, Kemp SA, Papa G, Datir R, Ferreira IATM, Marelli S, Harvey WT, Lytras S, Mohamed A, Gallo G, Thakur N, Collier DA, Mlcochova P, Duncan LM, Carabelli AM, Kenyon JC, Lever AM, De Marco A, Saliba C, Culap K, Cameroni E, Matheson NJ, Piccoli L, Corti D, James LC, Robertson DL, Bailey D, Gupta RK. Cell Rep. 2021 Jun 29;35(13):109292. doi: 10.1016/j.celrep.2021.109292. Epub 2021 Jun 8. PubMed |
| Effect of SARS-CoV-2 proteins on vascular permeability. Rauti R, Shahoha M, Leichtmann-Bardoogo Y, Nasser R, Paz E, Tamir R, Miller V, Babich T, Shaked K, Ehrlich A, Ioannidis K, Nahmias Y, Sharan R, Ashery U, Maoz BM. Elife. 2021 Oct 25;10. pii: 69314. doi: 10.7554/eLife.69314. PubMed |
| SARS-CoV-2 inhibits induction of the MHC class I pathway by targeting the STAT1-IRF1-NLRC5 axis. Yoo JS, Sasaki M, Cho SX, Kasuga Y, Zhu B, Ouda R, Orba Y, de Figueiredo P, Sawa H, Kobayashi KS. Nat Commun. 2021 Nov 15;12(1):6602. doi: 10.1038/s41467-021-26910-8. PubMed |
| Cell Surface SARS-CoV-2 Nucleocapsid Protein Modulates Innate and Adaptive Immunity. Lopez-Munoz AD, Kosik I, Holly J, Yewdell JW. bioRxiv. 2021 Dec 13. doi: 10.1101/2021.12.10.472169. PubMed |
| Inhalable SARS-CoV-2 Mimetic Particles Induce Pleiotropic Antigen Presentation. Lawanprasert A, Simonson AW, Sumner SE, Nicol MJ, Pimcharoen S, Kirimanjeswara GS, Medina SH. Biomacromolecules. 2022 Mar 14;23(3):1158-1168. doi: 10.1021/acs.biomac.1c01447. Epub 2022 Jan 26. PubMed |
| SARS-CoV-2 genomes from Saudi Arabia implicate nucleocapsid mutations in host response and increased viral load. Mourier T, Shuaib M, Hala S, Mfarrej S, Alofi F, Naeem R, Alsomali A, Jorgensen D, Subudhi AK, Ben Rached F, Guan Q, Salunke RP, Ooi A, Esau L, Douvropoulou O, Nugmanova R, Perumal S, Zhang H, Rajan I, Al-Omari A, Salih S, Shamsan A, Al Mutair A, Taha J, Alahmadi A, Khotani N, Alhamss A, Mahmoud A, Alquthami K, Dageeg A, Khogeer A, Hashem AM, Moraga P, Volz E, Almontashiri N, Pain A. Nat Commun. 2022 Feb 1;13(1):601. doi: 10.1038/s41467-022-28287-8. PubMed |
| Dynamic Ca(2+) sensitivity stimulates the evolved SARS-CoV-2 spike strain-mediated membrane fusion for enhanced entry. Singh P, Mukherji S, Basak S, Hoffmann M, Das DK. Cell Rep. 2022 Apr 19;39(3):110694. doi: 10.1016/j.celrep.2022.110694. Epub 2022 Apr 9. PubMed |
| Decline of humoral immune responses after natural SARS-CoV-2 infection can be efficiently reversed by vaccination. Oktelik FB, Yilmaz V, Gelmez MY, Akdeniz N, Pamukcu C, Sutlu T, Kose M, Tuzun E, Deniz G. Can J Microbiol. 2022 Aug 1;68(8):543-550. doi: 10.1139/cjm-2022-0003. Epub 2022 Jul 19. PubMed |
| Cell surface SARS-CoV-2 nucleocapsid protein modulates innate and adaptive immunity. Lopez-Munoz AD, Kosik I, Holly J, Yewdell JW. Sci Adv. 2022 Aug 5;8(31):eabp9770. doi: 10.1126/sciadv.abp9770. Epub 2022 Aug 3. PubMed |
| Thiopurines inhibit coronavirus Spike protein processing and incorporation into progeny virions. Pringle ES, Duguay BA, Bui-Marinos MP, Mulloy RP, Landreth SL, Desireddy KS, Dolliver SM, Ying S, Caddell T, Tooley TH, Slaine PD, Bearne SL, Falzarano D, Corcoran JA, Khaperskyy DA, McCormick C. PLoS Pathog. 2022 Sep 19;18(9):e1010832. doi: 10.1371/journal.ppat.1010832. eCollection 2022 Sep. PubMed |
| Cyclin D3 restricts SARS-CoV-2 envelope incorporation into virions and interferes with viral spread. Gupta RK, Mlcochova P. EMBO J. 2022 Nov 17;41(22):e111653. doi: 10.15252/embj.2022111653. Epub 2022 Oct 10. PubMed |
| SARS-CoV-2 escapes direct NK cell killing through Nsp1-mediated downregulation of ligands for NKG2D. Lee MJ, Leong MW, Rustagi A, Beck A, Zeng L, Holmes S, Qi LS, Blish CA. Cell Rep. 2022 Dec 27;41(13):111892. doi: 10.1016/j.celrep.2022.111892. Epub 2022 Dec 12. PubMed |
| Secreted ORF8 induces monocytic pro-inflammatory cytokines through NLRP3 pathways in patients with severe COVID-19. Wu X, Manske MK, Ruan GJ, Witter TL, Nowakowski KE, Abeykoon JP, Tang X, Yu Y, Gwin KA, Wu A, Taupin V, Bhardwaj V, Paludo J, Dasari S, Dong H, Ansell SM, Badley AD, Schellenberg MJ, Witzig TE. iScience. 2023 Jun 16;26(6):106929. doi: 10.1016/j.isci.2023.106929. Epub 2023 May 18. PubMed |
| Induction of antiviral gene expression by cyclosporine A, but not inhibition of cyclophilin A or B, contributes to its restriction of human coronavirus 229E infection in a lung epithelial cell line. Mamatis JE, Gallardo-Flores CE, Sangwan U, Tooley TH, Walsh T, Colpitts CC. Antiviral Res. 2023 Nov;219:105730. doi: 10.1016/j.antiviral.2023.105730. Epub 2023 Oct 5. PubMed |
Biomolecular condensates create phospholipid-enriched microenvironments.
Dumelie JG, Chen Q, Miller D, Attarwala N, Gross SS, Jaffrey SR.
Nat Chem Biol. 2023 Nov 16. doi: 10.1038/s41589-023-01474-4.
PubMed
Associated Plasmids |
| Differences in syncytia formation by SARS-CoV-2 variants modify host chromatin accessibility and cellular senescence via TP53. Lee JD, Menasche BL, Mavrikaki M, Uyemura MM, Hong SM, Kozlova N, Wei J, Alfajaro MM, Filler RB, Muller A, Saxena T, Posey RR, Cheung P, Muranen T, Heng YJ, Paulo JA, Wilen CB, Slack FJ. Cell Rep. 2023 Dec 26;42(12):113478. doi: 10.1016/j.celrep.2023.113478. Epub 2023 Nov 21. PubMed |
Simple visualization of submicroscopic protein clusters with a phase-separation-based fluorescent reporter.
Mumford TR, Rae D, Brackhahn E, Idris A, Gonzalez-Martinez D, Pal AA, Chung MC, Guan J, Rhoades E, Bugaj LJ.
Cell Syst. 2024 Feb 21;15(2):166-179.e7. doi: 10.1016/j.cels.2024.01.005. Epub 2024 Feb 8.
PubMed
Associated Plasmids |
| The Equal Neutralizing Effectiveness of BNT162b2, ChAdOx1 nCoV-19, and Sputnik V Vaccines in the Palestinian Population. Damour A, Faure M, Landrein N, Ragues J, Ardah N, Dhaidel H, Lafon ME, Wodrich H, Basha W. Vaccines (Basel). 2024 May 3;12(5):493. doi: 10.3390/vaccines12050493. PubMed |
| SARS-CoV-2 Accessory Protein ORF8 Targets the Dimeric IgA Receptor pIgR. Laprise F, Arduini A, Duguay M, Pan Q, Liang C. Viruses. 2024 Jun 22;16(7):1008. doi: 10.3390/v16071008. PubMed |
| Mapping the immunopeptidome of seven SARS-CoV-2 antigens across common HLA haplotypes. Braun A, Rowntree LC, Huang Z, Pandey K, Thuesen N, Li C, Petersen J, Littler DR, Raji S, Nguyen THO, Jappe Lange E, Persson G, Schantz Klausen M, Kringelum J, Chung S, Croft NP, Faridi P, Ayala R, Rossjohn J, Illing PT, Scull KE, Ramarathinam S, Mifsud NA, Kedzierska K, Sorensen AB, Purcell AW. Nat Commun. 2024 Aug 30;15(1):7547. doi: 10.1038/s41467-024-51959-6. PubMed |
| The SARS-CoV-2 nucleocapsid protein interferes with the full enzymatic activation of UPF1 and its interaction with UPF2. Nuccetelli V, Mghezzi-Habellah M, Deymier S, Roisin A, Gerard-Baraggia F, Rocchi C, Coureux PD, Gouet P, Cimarelli A, Mocquet V, Fiorini F. Nucleic Acids Res. 2025 Jan 11;53(2):gkaf010. doi: 10.1093/nar/gkaf010. PubMed |
| Chemokines simultaneously bind SARS-CoV-2 nucleocapsid protein RNA-binding and dimerization domains. Lopez-Munoz AD, Yewdell JW. Virol J. 2025 Mar 17;22(1):77. doi: 10.1186/s12985-025-02658-1. PubMed |
| SARS-CoV-2 N protein exerts antitumor effects in NSCLC by inducing DNA damage and augmenting chemotherapeutic sensitivity. Wang X, Cao F, Chen D, Bai Y, Cui X, Luo Z, Guo Y, Tong R, Wu M, Huang AL, Tang KF. Med Oncol. 2025 Jun 1;42(7):230. doi: 10.1007/s12032-025-02771-9. PubMed |
| Evolution of a fuzzy ribonucleoprotein complex in viral assembly. Zhao H, Li T, Hassan SA, Nguyen A, Datta SAK, Zhang G, Trent C, Czaja AM, Wu D, Aronova MA, Lai KK, Piszczek G, Leapman RD, Yewdell JW, Schuck P. bioRxiv [Preprint]. 2025 Nov 6:2025.04.26.650775. doi: 10.1101/2025.04.26.650775. PubMed |
Human protein interaction networks of ancestral and variant SARS-CoV-2 in organ-specific cells and bodily fluids.
Broderick K, Moutaoufik MT, Saccon T, Malty R, Amin S, Phanse S, Joseph TP, Zilocchi M, Hosseinnia A, Istace Z, Hajikarimlou M, Abrar S, Fisher J, Brassard R, Perera R, Kumar A, Aoki H, Rahmatbakhsh M, Jessulat M, Kobasa D, Dehne F, Prasad B, Gagarinova A, Joanne Lemieux M, Cochrane A, Houry WA, Aly KA, Golshani A, Babu M.
Nat Commun. 2025 Jul 1;16(1):5784. doi: 10.1038/s41467-025-60949-1.
PubMed
Associated Plasmids |
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