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LTRi
(Plasmid #15913)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15913 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Stratagene SURE Very low copy--TLD recommends megapreps
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

Resource Information

Depositor Comments

We engineered a tunable genetic switch called LTRi, which stands for Lac-Tet-RNAi. Gene expression is turned on by adding an inducer, isopropyl-β-thiogalactopyranoside (IPTG). See article for details.

Clone your gene of interest into the Transgene Module http://www.addgene.org/15916 and then move it into LTRi.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LTRi was a gift from James Collins (Addgene plasmid # 15913 ; http://n2t.net/addgene:15913 ; RRID:Addgene_15913)
  • For your References section:

    A tunable genetic switch based on RNAi and repressor proteins for regulating gene expression in mammalian cells. Deans TL, Cantor CR, Collins JJ. Cell. 2007 Jul 27. 130(2):363-72. 10.1016/j.cell.2007.05.045 PubMed 17662949