|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15913||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 8062
Vector typeMammalian Expression, Synthetic Biology
Growth in Bacteria
Growth instructionsStratagene SURE Very low copy--TLD recommends megapreps
Copy numberLow Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
We engineered a tunable genetic switch called LTRi, which stands for Lac-Tet-RNAi. Gene expression is turned on by adding an inducer, isopropyl-β-thiogalactopyranoside (IPTG). See article for details.
Clone your gene of interest into the Transgene Module http://www.addgene.org/15916 and then move it into LTRi.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LTRi was a gift from James Collins (Addgene plasmid # 15913 ; http://n2t.net/addgene:15913 ; RRID:Addgene_15913)
For your References section:A tunable genetic switch based on RNAi and repressor proteins for regulating gene expression in mammalian cells. Deans TL, Cantor CR, Collins JJ. Cell. 2007 Jul 27. 130(2):363-72. 10.1016/j.cell.2007.05.045 PubMed 17662949