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(Plasmid #164569)


Item Catalog # Description Quantity Price (USD)
Plasmid 164569 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4746
  • Total vector size (bp) 8370
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Spike (S-RRAR variant)
  • Species
    Severe acute respiratory syndrome coronavirus 2
  • Insert Size (bp)
  • GenBank ID
  • Tags / Fusion Proteins
    • HRV3C cleavage site (before tags) (C terminal on backbone)
    • 8X His tag (C terminal on backbone)
    • 2X Strep-Tag II (C terminal on backbone)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Jason McLellan
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pαH-S-RRAR was a gift from Priyamvada Acharya (Addgene plasmid # 164569 ; ; RRID:Addgene_164569)
  • For your References section:

    D614G Mutation Alters SARS-CoV-2 Spike Conformation and Enhances Protease Cleavage at the S1/S2 Junction. Gobeil SM, Janowska K, McDowell S, Mansouri K, Parks R, Manne K, Stalls V, Kopp MF, Henderson R, Edwards RJ, Haynes BF, Acharya P. Cell Rep. 2021 Jan 12;34(2):108630. doi: 10.1016/j.celrep.2020.108630. Epub 2020 Dec 26. 10.1016/j.celrep.2020.108630 PubMed 33417835