PurposetRNA synthetase/tRNA pair for the in vivo incorporation of N6-(trifluoroacetyl)-L-lysine (TFA-Lys) into proteins in E. coli in response to the amber (TAG) codon
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||177311||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2452
- Total vector size (bp) 3274
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert nametRNA synthetase
SpeciesMethanogenic archaeon ISO4-G1
Insert Size (bp)822
- Promoter T7
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer TAATACGACTCACTATAGGG
- 3′ sequencing primer GCTAGTTATTGCTCAGCGG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRSF-G1TFAKRS was a gift from Thomas Huber (Addgene plasmid # 177311 ; http://n2t.net/addgene:177311 ; RRID:Addgene_177311)
For your References section:Through-Space Scalar (19)F-(19)F Couplings between Fluorinated Noncanonical Amino Acids for the Detection of Specific Contacts in Proteins. Orton HW, Qianzhu H, Abdelkader EH, Habel EI, Tan YJ, Frkic RL, Jackson CJ, Huber T, Otting G. J Am Chem Soc. 2021 Nov 15. doi: 10.1021/jacs.1c10104. 10.1021/jacs.1c10104 PubMed 34780162