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Addgene

M8
(Plasmid #180253)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180253 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTK1-TPA
  • Backbone size w/o insert (bp) 4967
  • Total vector size (bp) 5638
  • Vector type
    Mammalian Expression ; vaccine vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Synthetic gene encoding for 8 neonatigens specific for MC38 mouse cancer cells
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    696
  • Mutation
    the polyepitope vaccine encodes for mutated sequence expressed in the MC38 tumor model
  • GenBank ID
    NM_001359282.1
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer GAGGGCAGTGTAGTCTGAGCAGTACTCG
  • 3′ sequencing primer GGACAGTGGGAGTGGCACCTTCC
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M8 was a gift from Fabio Palombo (Addgene plasmid # 180253 ; http://n2t.net/addgene:180253 ; RRID:Addgene_180253)

  • For your References section:

    Neoantigen cancer vaccine augments anti-CTLA-4 efficacy. Salvatori E, Lione L, Compagnone M, Pinto E, Conforti A, Ciliberto G, Aurisicchio L, Palombo F. NPJ Vaccines. 2022 Feb 2;7(1):15. doi: 10.1038/s41541-022-00433-9. 10.1038/s41541-022-00433-9 PubMed 35110563
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