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pBBR1-P2-SpCas9
(Plasmid #232354)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232354 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBBR1-MCS
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Cas9
  • Species
    S. pyogenes
  • Promoter promoter P2 from S. marcescens HBQA7

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    sacB

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBBR1-P2-SpCas9 was a gift from Yujie Cai (Addgene plasmid # 232354 ; http://n2t.net/addgene:232354 ; RRID:Addgene_232354)

  • For your References section:

    Construction of a CRISPR-Cas9-Based Genetic Editing Tool for Serratia marcescens Using a Stationary Phase Promoter and Its Application in Putrescine Production. Gou L, Liu D, Fan TP, Deng H, Cai Y. Biotechnol Bioeng. 2025 May;122(5):1233-1244. doi: 10.1002/bit.28949. Epub 2025 Feb 5. 10.1002/bit.28949 PubMed 39910976
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