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Addgene

pAAV-P3-IgKL-SpyCatcher003-SEP
(Plasmid #234541)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234541 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-P3 (Addgene plasmid #183460)
  • Backbone manufacturer
    VVF (p438) https://vvf.ethz.ch/
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IgKL-SpyCatcher003-SEP
  • Alt name
    IgKL-SC3-SEP
  • Alt name
    (superecliptic pHluorin)
  • Species
    Other
  • Mutation
    None
  • Promoter P3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.03.18.643859 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-P3-IgKL-SpyCatcher003-SEP was a gift from Hajime Hirase (Addgene plasmid # 234541 ; http://n2t.net/addgene:234541 ; RRID:Addgene_234541)

  • For your References section:

    Functional and structural profiling of circulation via genetically encoded modular fluorescent probes. Vittani M, Lee AB, Wang X, Hiraoka Y, Konno A, Knak PG, Kusk P, Nagao M, Asiminas A, Courtin J, Putranto MF, Nasu Y, Tsuno S, Ueda K, Osuga Y, Tsuboi T, Bienvenu T, Terunuma M, Hirai H, Nedergaard M, Tanaka K, Hirase H. bioRxiv 2025.03.18.643859 10.1101/2025.03.18.643859
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