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Addgene

pcDNA3.1 NPY pHCitron
(Plasmid #235175)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235175 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418) ; Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NPY-pHCitron
  • Species
    H. sapiens (human)
  • Entrez Gene
    NPY (a.k.a. PYY4)
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 NPY pHCitron was a gift from Kate White (Addgene plasmid # 235175 ; http://n2t.net/addgene:235175 ; RRID:Addgene_235175)
  • For your References section:

    Decoding Insulin Secretory Granule Maturation Using Genetically Encoded pH Sensors. Lin W, Tseng K, Fraser SE, Junge J, White KL. ACS Sens. 2024 Nov 22;9(11):6032-6039. doi: 10.1021/acssensors.4c01885. Epub 2024 Nov 6. 10.1021/acssensors.4c01885 PubMed 39504473