pcDNA3.1 NPY pHCitron
(Plasmid
#235175)
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PurposePlasmid expressing NPY tag with pH sensor, pHCitron (pH-Lemon with mCitrine), in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 235175 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418) ; Kanamycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNPY-pHCitron
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SpeciesH. sapiens (human)
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Entrez GeneNPY (a.k.a. PYY4)
- Promoter CMV
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer CMV
- 3′ sequencing primer bGH (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1 NPY pHCitron was a gift from Kate White (Addgene plasmid # 235175 ; http://n2t.net/addgene:235175 ; RRID:Addgene_235175) -
For your References section:
Decoding Insulin Secretory Granule Maturation Using Genetically Encoded pH Sensors. Lin W, Tseng K, Fraser SE, Junge J, White KL. ACS Sens. 2024 Nov 22;9(11):6032-6039. doi: 10.1021/acssensors.4c01885. Epub 2024 Nov 6. 10.1021/acssensors.4c01885 PubMed 39504473