PEtracer Lentiviral Libraries
(Pooled Libraries #238546, #238547, #238548)

• Purpose:

  The PEtracer lentiviral puromycin and blasticidin barcode libraries contain selectable markers that encode ground truth static marks for phylogenetic reconstructions.

  The LTC_v2 library encodes a library of target sites used for the PEtracer technology.

• Vector Backbone:

  • Library #238546: lenti_Puro_10N_barcode backbone (DNA, 52 KB)
  • Library #238547: lenti_Blast_10N_barcode backbone (DNA, 48 KB)
  • Library #238548: lenti_LTC_v2 backbone (DNA, 64 KB)

• Depositing Labs: Jonathan Weissman
• Publication: Koblan et al. Science. 2025 Jul 24;eadx3800. doi: 10.1126/science.adx3800.

Library Details

• Number of elements:
  
  • Library #238546: ~100,000–150,000
  • Library #238547: ~100,000–150,000
  • Library #238548: 2,170
• Lentiviral generation: 3rd

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

• Volume: 20 µl
• Concentration: 100 ng/µl

Resource Information

• Protocols:
  • Library Amplification Protocol (PDF, 407 KB)
  • Library Cloning and Sequencing Protocol (DOCX, 20 KB)
• Depositor Data:
  • Library Representation Plots (DOCX, 455 KB)
• Scripts:
  • Alignment Script (SLURM, 2 KB)
  • Library Representation Script (PY, 8 KB)

Terms and Licenses

Academic/Nonprofit Terms:

• UBMTA

Industry Terms:

• Not Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.

Depositor Comments

Representation for the lenti_LTC_v2 library was determined by aligning fastq files to the 2,171 target site sequences ordered from Twist Bioscience using the alignment script and then running the library representation script (see Scripts under Resource Information). Representation for the puromycin and blasticidin barcode libraries was directly calculated from the fastq files by running the library representation script with match=TGTGGGAGGGCTAAC{BC}GTTTAAACCCGCTGA for blasticidin and match=AGCCTGGCGCCTGAC{BC}GTTTAAACCCGCTGA for puromycin.