Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24095||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepLentiLox 3.7
- Backbone size w/o insert (bp) 7650
Growth in Bacteria
Gene/Insert nameGlucocorticoid Receptor
SpeciesR. norvegicus (rat)
Insert Size (bp)80
Entrez GeneNr3c1 (a.k.a. GR, Gcr, Grl)
/ Fusion Protein
- GFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HpaI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer TRC-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
We designed an shRNA targeting the rat GR sequence in the pLentiLox3.7 (ATCC) plasmid carrying a GFP reporter: forward sequence, 5′-tgcgggagaagatgatccattcttcaagagagaatggatcatcttctcccgcttttttgaattc; reverse sequence, 5′-tcgagaattcaaaaaagcgggagaagatgatccattctctcttgaagaatggatcatcttctcccgca.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:shRNA GR was a gift from Moses Chao (Addgene plasmid # 24095 ; http://n2t.net/addgene:24095 ; RRID:Addgene_24095)
For your References section:Activation of Trk neurotrophin receptors by glucocorticoids provides a neuroprotective effect. Jeanneteau F, Garabedian MJ, Chao MV. Proc Natl Acad Sci U S A. 2008 Mar 25. 105(12):4862-7. 10.1073/pnas.0709102105 PubMed 18347336