pAAV GFAP CaBLAM
(Plasmid #244228)

• Purpose: Bioluminescent reporter for calcium signaling in astrocytes
• Depositing Lab: Ute Hochgeschwender
• Publication: Lambert et al Nat Methods. 2025 Dec 2. doi: 10.1038/s41592-025-02972-0.

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 4835
• Total vector size (bp): 6605
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: mNeonGreen
• Alt name: mNG
• Species: Branchiostoma lanceolatum
• Insert Size (bp): 678

Gene/Insert 2

• Gene/Insert name: CaBLAM
• Species: Synthetic
• Insert Size (bp): 1089

Resource Information

• Supplemental Documents:
  • pAAV GFAP CaBLAM.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2023.06.25.546478 for bioRxiv preprint.