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Addgene

pAAV GFAP CaBLAM
(Plasmid #244228)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244228 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 4835
  • Total vector size (bp) 6605
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    mNeonGreen
  • Alt name
    mNG
  • Species
    Branchiostoma lanceolatum
  • Insert Size (bp)
    678

Gene/Insert 2

  • Gene/Insert name
    CaBLAM
  • Species
    Synthetic
  • Insert Size (bp)
    1089

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.06.25.546478 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV GFAP CaBLAM was a gift from Ute Hochgeschwender (Addgene plasmid # 244228 ; http://n2t.net/addgene:244228 ; RRID:Addgene_244228)
  • For your References section:

    CaBLAM! A high-contrast bioluminescent Ca (2+) indicator derived from an engineered Oplophorus gracilirostris luciferase. Lambert GG, Crespo EL, Murphy J, Boassa D, Luong S, Hu J, Sprecher B, Tree MO, Orcutt R, Heydari D, Bell AB, Torreblanca Zanca A, Hakimi A, Lipscombe D, Moore CI, Hochgeschwender U, Shaner NC. bioRxiv. 2023 Jun 26:2023.06.25.546478. doi: 10.1101/2023.06.25.546478. Preprint. 10.1101/2023.06.25.546478 PubMed 37425712