pAAV GFAP CaBLAM
(Plasmid
#244228)
-
PurposeBioluminescent reporter for calcium signaling in astrocytes
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 244228 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
-
Vector backbonepAAV
- Backbone size w/o insert (bp) 4835
- Total vector size (bp) 6605
-
Vector typeAAV
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberLow Copy
Gene/Insert 1
-
Gene/Insert namemNeonGreen
-
Alt namemNG
-
SpeciesBranchiostoma lanceolatum
-
Insert Size (bp)678
Gene/Insert 2
-
Gene/Insert nameCaBLAM
-
SpeciesSynthetic
-
Insert Size (bp)1089
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.06.25.546478 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAAV GFAP CaBLAM was a gift from Ute Hochgeschwender (Addgene plasmid # 244228 ; http://n2t.net/addgene:244228 ; RRID:Addgene_244228) -
For your References section:
CaBLAM! A high-contrast bioluminescent Ca (2+) indicator derived from an engineered Oplophorus gracilirostris luciferase. Lambert GG, Crespo EL, Murphy J, Boassa D, Luong S, Hu J, Sprecher B, Tree MO, Orcutt R, Heydari D, Bell AB, Torreblanca Zanca A, Hakimi A, Lipscombe D, Moore CI, Hochgeschwender U, Shaner NC. bioRxiv. 2023 Jun 26:2023.06.25.546478. doi: 10.1101/2023.06.25.546478. Preprint. 10.1101/2023.06.25.546478 PubMed 37425712