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pBAD-ColH-HylB
(Plasmid #248631)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248631 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBAD/His A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3959
  • Total vector size (bp) 10081
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Myc-tagged bacterial collagenase, ColH, and His-tagged hyaluronidase, HylB
  • Species
    Synthetic
  • Insert Size (bp)
    6115
  • GenBank ID
  • Promoter araBAD
  • Tags / Fusion Proteins
    • Myc (C terminal on insert)
    • His (C terminal on insert)

Cloning Information

  • Cloning method Gene Synthesis
  • 5′ sequencing primer AAC TCT CTA CTG TTT CTC CAT ACC CGT TT
  • 3′ sequencing primer TGT ATC AGG CTG AAA ATC TTC TCT CAT CC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD-ColH-HylB was a gift from Edwin Manuel (Addgene plasmid # 248631 ; http://n2t.net/addgene:248631 ; RRID:Addgene_248631)

  • For your References section:

    Tumor-Colonizing E. coli Expressing Both Collagenase and Hyaluronidase Enhances Therapeutic Efficacy of Gemcitabine in Pancreatic Cancer Models. Avsharian LC, Loganathan S, Ebelt ND, Shalamzari AF, Rodarte Munoz I, Manuel ER. Biomolecules. 2024 Nov 17;14(11):1458. doi: 10.3390/biom14111458. 10.3390/biom14111458 PubMed 39595636
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