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pMSP1E3D1_mBlueberry2
(Plasmid #248930)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 248930 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET-28a(+)
  • Backbone manufacturer
    GenScript
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MSP1E3D1_mBlueberry2
  • Species
    E. coli
  • Insert Size (bp)
    1548
  • Promoter T7 Promoter
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nco1 (not destroyed)
  • 3′ cloning site Hind III (not destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Stephen Sligar (Addgene #20066), and the fluorescent protein sequence was derived from https://www.fpbase.org/protein/mblueberry2/ (DOI: 10.1021/bi700199g).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor recommends growing plasmid in the E. coli strain BL21 (DE3).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSP1E3D1_mBlueberry2 was a gift from Michael Marty (Addgene plasmid # 248930 ; http://n2t.net/addgene:248930 ; RRID:Addgene_248930)
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