pLKO.1 RagB shRNA #2
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26628||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepLKO.1 puro
Backbone manufacturerAvailable at Addgene (#8453)
- Backbone size w/o insert (bp) 7000
Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameRagB shRNA
SpeciesH. sapiens (human)
Entrez GeneRRAGB (a.k.a. RAGB, bA465E19.1)
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer LKO.1 5' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
shRNA directed against RagB. Target sequence: 5'-GCCTCTTTCCAGAGTATGGAA-3'. Sequence from the website of the RNAi consortium at the Broad institute.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLKO.1 RagB shRNA #2 was a gift from David Sabatini (Addgene plasmid # 26628 ; http://n2t.net/addgene:26628 ; RRID:Addgene_26628)
For your References section:The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled L, Sabatini DM. Science. 2008 Jun 13. 320(5882):1496-501. 10.1126/science.1157535 PubMed 18497260