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pAM1956
(Plasmid #40251)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40251 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFPmut2
  • Species
    Anabaena sp. Strain PCC 7120
  • Insert Size (bp)
    800
  • Promoter n/a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site n/a (unknown if destroyed)
  • 3′ cloning site n/a (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Contain promoterless gfp from pKEN2-GFPmut2 inserted into pAM505.

HindIII-Klenow fill-SacI fragment of gfp is inserted into SmaI-SacI digested pAM505. This is a control of gfp without a promoter. The multiple cloning site upstream the gfp gene includes: SalI, SacI, KpnI,Asp718, XmaI, and SmaI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM1956 was a gift from Susan Golden (Addgene plasmid # 40251 ; http://n2t.net/addgene:40251 ; RRID:Addgene_40251)
  • For your References section:

    Heterocyst pattern formation controlled by a diffusible peptide. Yoon HS, Golden JW. Science. 1998 Oct 30;282(5390):935-8. 10.1126/science.282.5390.935 PubMed 9794762