|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40251||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Total vector size (bp) 11000
Vector typeCyanobacteria cloning vector
Growth in Bacteria
SpeciesAnabaena sp. Strain PCC 7120
Insert Size (bp)800
- Promoter n/a
- Cloning method Restriction Enzyme
- 5′ cloning site n/a (unknown if destroyed)
- 3′ cloning site n/a (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
Contain promoterless gfp from pKEN2-GFPmut2 inserted into pAM505.
HindIII-Klenow fill-SacI fragment of gfp is inserted into SmaI-SacI digested pAM505. This is a control of gfp without a promoter. The multiple cloning site upstream the gfp gene includes: SalI, SacI, KpnI,Asp718, XmaI, and SmaI.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAM1956 was a gift from Susan Golden (Addgene plasmid # 40251 ; http://n2t.net/addgene:40251 ; RRID:Addgene_40251)
For your References section:Heterocyst pattern formation controlled by a diffusible peptide. Yoon HS, Golden JW. Science. 1998 Oct 30;282(5390):935-8. 10.1126/science.282.5390.935 PubMed 9794762