pHR-scFv-GCN4-sfGFP-GB1-dWPRE Citations (18)

Originally described in: A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging.
Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. PubMed Journal

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Articles Citing pHR-scFv-GCN4-sfGFP-GB1-dWPRE

Articles
Visualization of single endogenous polysomes reveals the dynamics of translation in live human cells. Pichon X, Bastide A, Safieddine A, Chouaib R, Samacoits A, Basyuk E, Peter M, Mueller F, Bertrand E. J Cell Biol. 2016 Sep 12;214(6):769-81. doi: 10.1083/jcb.201605024. Epub 2016 Sep 5. PubMed
Imaging Translation Dynamics of Single mRNA Molecules in Live Cells. Ruijtenberg S, Hoek TA, Yan X, Tanenbaum ME. Methods Mol Biol. 2018;1649:385-404. doi: 10.1007/978-1-4939-7213-5_26. PubMed
Single-Molecule Quantification of Translation-Dependent Association of mRNAs with the Endoplasmic Reticulum. Voigt F, Zhang H, Cui XA, Triebold D, Liu AX, Eglinger J, Lee ES, Chao JA, Palazzo AF. Cell Rep. 2017 Dec 26;21(13):3740-3753. doi: 10.1016/j.celrep.2017.12.008. PubMed Associated Plasmids phage UbiC SNAP-KDEL pSF4 TetCMV intron 4xGCN4 Renilla FKBP STOP 24xMS2v5 SV40 CTE polyA phage UbiC scAB-GFP phage UbiC NLS HA stdMCP stdHalo pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA More ...
Comparison and optimization of CRISPR/dCas9/gRNA genome-labeling systems for live cell imaging. Hong Y, Lu G, Duan J, Liu W, Zhang Y. Genome Biol. 2018 Mar 22;19(1):39. doi: 10.1186/s13059-018-1413-5. PubMed
Late Endosomes Act as mRNA Translation Platforms and Sustain Mitochondria in Axons. Cioni JM, Lin JQ, Holtermann AV, Koppers M, Jakobs MAH, Azizi A, Turner-Bridger B, Shigeoka T, Franze K, Harris WA, Holt CE. Cell. 2019 Jan 10;176(1-2):56-72.e15. doi: 10.1016/j.cell.2018.11.030. Epub 2019 Jan 3. PubMed
Live-Cell Single RNA Imaging Reveals Bursts of Translational Frameshifting. Lyon K, Aguilera LU, Morisaki T, Munsky B, Stasevich TJ. Mol Cell. 2019 Jul 11;75(1):172-183.e9. doi: 10.1016/j.molcel.2019.05.002. Epub 2019 Jun 6. PubMed Associated Plasmids Multi-frame tag smHA multi-frame tag
A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo. Zhao N, Kamijo K, Fox PD, Oda H, Morisaki T, Sato Y, Kimura H, Stasevich TJ. Nat Commun. 2019 Jul 3;10(1):2947. doi: 10.1038/s41467-019-10846-1. PubMed Associated Plasmids pCMV-15F11-HA-mEGFP pCMV-15F11-HA-mCh pCMV-15F11-HA-Halo pET23b-15F11-HA-mEGFP-6xHis pET23b-2E2-HA-mEGFP-6xHis More ...
New Generations of MS2 Variants and MCP Fusions to Detect Single mRNAs in Living Eukaryotic Cells. Pichon X, Robert MC, Bertrand E, Singer RH, Tutucci E. Methods Mol Biol. 2020;2166:121-144. doi: 10.1007/978-1-0716-0712-1_7. PubMed
Live Imaging of RNA Transport and Translation in Xenopus Retinal Axons. Lin JQ, Cioni JM. Methods Mol Biol. 2022;2431:49-69. doi: 10.1007/978-1-0716-1990-2_3. PubMed
Enhanced single RNA imaging reveals dynamic gene expression in live animals. Hu Y, Xu J, Gao E, Fan X, Wei J, Ye B, Xu S, Ma W. Elife. 2023 Mar 3;12:e82178. doi: 10.7554/eLife.82178. PubMed Associated Plasmids pSX2719 pSX2720 pSX2739 pcDNA-tdMCP-24xGCN4 pcDNA-tdMCP-12xGCN4 More ...
Maintenance of a short-lived protein required for long-term memory involves cycles of transcription and local translation. Das S, Lituma PJ, Castillo PE, Singer RH. Neuron. 2023 Jul 5;111(13):2051-2064.e6. doi: 10.1016/j.neuron.2023.04.005. Epub 2023 Apr 25. PubMed
Subcytoplasmic location of translation controls protein output. Horste EL, Fansler MM, Cai T, Chen X, Mitschka S, Zhen G, Lee FCY, Ule J, Mayr C. Mol Cell. 2023 Dec 21;83(24):4509-4523.e11. doi: 10.1016/j.molcel.2023.11.025. PubMed Associated Plasmids MCP-mCherry MCP-mCherry-TIAL1 MCP-mCherry-TIS11B MCP-mCherry-SEC61B
Optogenetic Strategies for Optimizing the Performance of Phospholipids Biosensors. Yao Y, Lou X, Jin L, Sun W, Liu J, Chen Y, Cheng S, Zhao T, Ke S, Zhang L, Xu Y, He L, Li H. Adv Sci (Weinh). 2024 Sep;11(36):e2403026. doi: 10.1002/advs.202403026. Epub 2024 Jul 29. PubMed
Unidirectional recruitment between MeCP2 and KSHV-encoded LANA revealed by CRISPR/Cas9 recruitment assay. Lavi I, Bhattacharya S, Awase A, Orgil O, Avital N, Journo G, Gurevich V, Shamay M. PLoS Pathog. 2025 Mar 10;21(3):e1012972. doi: 10.1371/journal.ppat.1012972. eCollection 2025 Mar. PubMed
Measuring and manipulating localized translation of erm-1 in the C. elegans embryo. van der Salm E, Koelewijn E, Schroeder M, van der Maas E, Jarosinska O, Eeken M, Ruijtenberg S. Development. 2025 May 15;152(10):dev204435. doi: 10.1242/dev.204435. Epub 2025 May 19. PubMed Associated Plasmids pSRG12 (eft-3p::24xGCN4::t2a::tagbfp::h2b::tbb-2 3’UTR) pSRG11 (eft-3p::scfv(glo)::gfp(smu-1 introns)::tbb-2 3’UTR)
Beyond Trypanosoma cruzi: LINE-1 Activation as a Driver of Chronic Inflammation in Chagas Disease. Dias M, Moraes A, Shiroma T, Pessoa V, Ermoges A, Vital T, Hagstrom L, de Sousa D, de Castro M, Dallago B, Bastos IMD, Nitz N, Hecht M. Int J Mol Sci. 2025 May 8;26(10):4466. doi: 10.3390/ijms26104466. PubMed
Maintenance of neuronal TDP-43 expression requires axonal lysosome transport. Ryan VH, Lawton S, Reyes JF, Hawrot J, Frankenfield AM, Seddighi S, Ramos DM, Epstein J, Faghri F, Johnson NL, Zou J, Kampmann M, Replogle J, Qi YA, Yuan H, Johnson K, Maric D, Hao L, Nalls MA, Ward ME. Elife. 2025 Sep 19;14:RP104057. doi: 10.7554/eLife.104057. PubMed
BRIGHT Enables High-SNR Live-Cell Imaging of Non-Repetitive Sequences via Bivalent Fluorescent Nanobody-Mediated Cascade-Dependent Illumination. Feng L, Huang T, Han Y, Tian M, Wang D, Mei Z, Ma Y, Ma Y, Li J, Zhang R. Adv Sci (Weinh). 2026 Jan;13(2):e13014. doi: 10.1002/advs.202513014. Epub 2025 Oct 17. PubMed

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