pSF4 CMV 5TOP intron renilla TRICK CTE polyA
(Plasmid #64544)

• Purpose: 5′ TOP TRICK reporter mRNA
• Depositing Lab: Jeffrey Chao
• Publication: Halstead et al Science. 2015 Mar 20;347(6228):1367-671. doi: 10.1126/science.aaa3380.

Backbone

• Vector backbone: pSF4
• Total vector size (bp): 9912
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 12x PP7 stem-loops
• Species: Synthetic
• Promoter: Tetracycline-inducible promoter and 5′ UTR of human RPL32 from rpL32-β-globin
• Tags / Fusion Proteins:
  • 5' TOP (5′ terminal oligopyrimidine motifs) (N terminal on insert)
  • Renilla luciferase (N terminal on insert)
  • 24xMS2 stem loops (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: TRE-F

Resource Information

• Supplemental Documents:
  • pSF4 CMV 5TOP intron renilla TRICK CTE polyA genbank

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry