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pAAVS1-NDi-CRISPRi (Gen2) Citations (12)

Originally described in: CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs.
Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, Weissman JS, Qi LS, So PL, Conklin BR Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 10.1016/j.stem.2016.01.022. Epub 2016 Mar 10. PubMed Journal

Articles Citing pAAVS1-NDi-CRISPRi (Gen2)

Articles
OSMR controls glioma stem cell respiration and confers resistance of glioblastoma to ionizing radiation. Sharanek A, Burban A, Laaper M, Heckel E, Joyal JS, Soleimani VD, Jahani-Asl A. Nat Commun. 2020 Aug 17;11(1):4116. doi: 10.1038/s41467-020-17885-z. PubMed
Transcriptional suppression of ribosomal DNA with phase separation. Ide S, Imai R, Ochi H, Maeshima K. Sci Adv. 2020 Oct 14;6(42). pii: 6/42/eabb5953. doi: 10.1126/sciadv.abb5953. Print 2020 Oct. PubMed

Associated Plasmids
Pluripotent stem cell model of early hematopoiesis in Down syndrome reveals quantitative effects of short-form GATA1 protein on lineage specification. Matsuo S, Nishinaka-Arai Y, Kazuki Y, Oshimura M, Nakahata T, Niwa A, Saito MK. PLoS One. 2021 Mar 29;16(3):e0247595. doi: 10.1371/journal.pone.0247595. eCollection 2021. PubMed
Deletion of lncRNA XACT does not change expression dosage of X-linked genes, but affects differentiation potential in hPSCs. Motosugi N, Okada C, Sugiyama A, Kawasaki T, Kimura M, Shiina T, Umezawa A, Akutsu H, Fukuda A. Cell Rep. 2021 Jun 8;35(10):109222. doi: 10.1016/j.celrep.2021.109222. PubMed
A functional genetic toolbox for human tissue-derived organoids. Sun D, Evans L, Perrone F, Sokleva V, Lim K, Rezakhani S, Lutolf M, Zilbauer M, Rawlins EL. eLife. 2021 Oct 6;10. pii: 67886. doi: 10.7554/eLife.67886. PubMed

Associated Plasmids
CRISPR interference interrogation of COPD GWAS genes reveals the functional significance of desmoplakin in iPSC-derived alveolar epithelial cells. Werder RB, Liu T, Abo KM, Lindstrom-Vautrin J, Villacorta-Martin C, Huang J, Hinds A, Boyer N, Bullitt E, Liesa M, Silverman EK, Kotton DN, Cho MH, Zhou X, Wilson AA. Sci Adv. 2022 Jul 15;8(28):eabo6566. doi: 10.1126/sciadv.abo6566. Epub 2022 Jul 13. PubMed

Associated Plasmids
Organoid modeling of human fetal lung alveolar development reveals mechanisms of cell fate patterning and neonatal respiratory disease. Lim K, Donovan APA, Tang W, Sun D, He P, Pett JP, Teichmann SA, Marioni JC, Meyer KB, Brand AH, Rawlins EL. Cell Stem Cell. 2023 Jan 5;30(1):20-37.e9. doi: 10.1016/j.stem.2022.11.013. Epub 2022 Dec 8. PubMed

Associated Plasmids
Epigenetic and transcriptional regulations prime cell fate before division during human pluripotent stem cell differentiation. Madrigal P, Deng S, Feng Y, Militi S, Goh KJ, Nibhani R, Grandy R, Osnato A, Ortmann D, Brown S, Pauklin S. Nat Commun. 2023 Jan 25;14(1):405. doi: 10.1038/s41467-023-36116-9. PubMed
Loss of TJP1 disrupts gastrulation patterning and increases differentiation toward the germ cell lineage in human pluripotent stem cells. Vasic I, Libby ARG, Maslan A, Bulger EA, Zalazar D, Krakora Compagno MZ, Streets A, Tomoda K, Yamanaka S, McDevitt TC. Dev Cell. 2023 Aug 21;58(16):1477-1488.e5. doi: 10.1016/j.devcel.2023.05.019. Epub 2023 Jun 23. PubMed
BRD9-SMAD2/3 Orchestrates Stemness and Tumorigenesis in Pancreatic Ductal Adenocarcinoma. Feng Y, Cai L, Pook M, Liu F, Chang CH, Mouti MA, Nibhani R, Militi S, Dunford J, Philpott M, Fan Y, Fan GC, Liu Q, Qi J, Wang C, Hong W, Morgan H, Wang M, Sadayappan S, Jegga AG, Oppermann U, Wang Y, Huang W, Jiang L, Pauklin S. Gastroenterology. 2024 Jan;166(1):139-154. doi: 10.1053/j.gastro.2023.09.021. Epub 2023 Sep 21. PubMed
The pRb/RBL2-E2F1/4-GCN5 axis regulates cancer stem cell formation and G0 phase entry/exit by paracrine mechanisms. Chang CH, Liu F, Militi S, Hester S, Nibhani R, Deng S, Dunford J, Rendek A, Soonawalla Z, Fischer R, Oppermann U, Pauklin S. Nat Commun. 2024 Apr 27;15(1):3580. doi: 10.1038/s41467-024-47680-z. PubMed
Elevated p16Ink4a Expression Enhances Tau Phosphorylation in Neurons Differentiated From Human-Induced Pluripotent Stem Cells. Holloway K, Neherin K, Song Y, Sato K, Houston A, Chen F, Ding L, Zhang H. Aging Cell. 2025 May;24(5):e14472. doi: 10.1111/acel.14472. Epub 2025 Jan 5. PubMed

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