pDZ2087
(Plasmid #92414)

• Purpose: Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli
• Depositing Lab: David Waugh
• Publication: Raran-Kurussi et al Methods Mol Biol. 2017;1586:221-230. doi: 10.1007/978-1-4939-6887-9_14.

Backbone

• Vector backbone: pMalC2
• Backbone manufacturer: New England Biolabs
• Total vector size (bp): 7393
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): BL21-RIL
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TEV protease
• Species: tobacco etch virus
• Mutation: L56V/S135G/S219V
• Promoter: tac
• Tag / Fusion Protein:
  • MBP (self cleaving) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: MBP

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

BL21-RIL strain is chloramphenicol resistant.