Engineering the AAVS1 locus for consistent and scalable transgene expression in human iPSCs and their differentiated derivatives.

Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K
Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 10.1016/j.ymeth.2015.12.012. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
80487	pAAVS1-Nst-MCS	donor vector for AAVS1 targeting (neomycin selection) with multiple cloning site
80488	pAAVS1-P-MCS	donor vector for AAVS1 targeting (puromycin selection) with multiple cloning site
80489	pAAVS1-Nst-CAG-DEST	Gateway Destination donor vector for AAVS1 targeting (puromycin selection)
80490	pAAVS1-P-CAG-DEST	Gateway Destination donor vector for AAVS1 targeting (puromycin selection)
80491	pAAVS1-P-CAG-GFP	donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expression
80492	pAAVS1-P-CAG-mCh	donor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expression
80493	pAAVS1-P-CAG-GFPluc2	donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expression
80494	pXAT2	AAVS1 sgRNA expression vector
80495	pTALdNC-AAVS1_T1	AAVS1 TALEN expression vector (Left)
80496	pTALdNC-AAVS1_T2	AAVS1 TALEN expression vector (Right)