Engineered Cpf1 variants with altered PAM specificities.
Gao L, Cox DBT, Yan WX, Manteiga JC, Schneider MW, Yamano T, Nishimasu H, Nureki O, Crosetto N, Zhang F
Nat Biotechnol. 2017 Jun 5. doi: 10.1038/nbt.3900.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 89351 | pcDNA3.1-hAsCpf1(TYCV) (pY210) | Expresses humanized AsCpf1 variant that recognizes TYCV PAMs |
| 89352 | pAsCpf1(TYCV)(BB) (pY211) | Expresses humanized AsCpf1 TYCV PAM variant and crRNA guide |
| 89353 | pcDNA3.1-hAsCpf1(TATV) (pY220) | Expresses humanized AsCpf1 variant that recognizes TATV PAMs |
| 89354 | pAsCpf1(TATV)(BB) (pY221) | Expresses humanized AsCpf1 TATV PAM variant and crRNA guide |
| 89355 | pcDNA3.1-hLbCpf1(TYCV) (pY230) | Expresses humanized LbCpf1 variant that recognizes TYCV PAMs |